Journal article
Differential cell adhesion to vocal fold extracellular matrix constituents
Matrix biology, Vol.25(4), pp.240-251
05/01/2006
DOI: 10.1016/j.matbio.2006.01.004
PMID: 16531030
Abstract
The human vocal folds are a complex layering of cells and extracellular matrix. Vocal fold extracellular matrix uniquely contributes to the biomechanical viscoelasticity required for human phonation. We investigated the adhesion of vocal fold stellate cells, a novel cell type first cultured by our laboratory, and fibroblasts to eight vocal fold extracellular matrix components: elastin, decorin, fibronectin, hyaluronic acid, laminin and collagen types I, III and IV. Our data demonstrate that these cells adhere differentially to said substrates at 5 to 120 min. Cells were treated with hyaluronidase and Y-27632, a p160ROCK-specific inhibitor, to test the role of pericellular hyaluronan and Rho-ROCK activation in early and mature adhesion. Reduced adhesion resulted; greater inhibition of fibroblast adhesion was observed. We modulated the fibronectin affinity exhibited by both cell types using Nimesulide, an inhibitor of fibronectin integrin receptors α5β1 and αvβ3. Our results are important in understanding vocal fold pathologies, wound healing, scarring, and in developing an accurate organotypic model of the vocal folds.
Details
- Title: Subtitle
- Differential cell adhesion to vocal fold extracellular matrix constituents
- Creators
- Tannin J. Fuja - University of IowaErin M. Ostrem - National Center for Voice and Speech, Department of Speech Pathology and Audiology, The University of Iowa, Iowa City, IA 52242, USAMegan N. Probst-Fuja - University of IowaIngo R. Titze - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Matrix biology, Vol.25(4), pp.240-251
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.matbio.2006.01.004
- PMID
- 16531030
- ISSN
- 0945-053X
- eISSN
- 1569-1802
- Number of pages
- 12
- Language
- English
- Date published
- 05/01/2006
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719754502771
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