Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras

Nancy Alvarez-Corrales; Raija K Ahmed; Carol A Rodriguez; Kithiganahalli N Balaji; Rebeca Rivera; Ramakrishna Sompallae; Nalini K Vudattu; Sven E Hoffner; Alimuddin Zumla; Lelany Pineda-Garcia; Markus Maeurer

doi:10.1186/1471-2334-13-125

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Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras

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Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras

Nancy Alvarez-Corrales, Raija K Ahmed, Carol A Rodriguez, Kithiganahalli N Balaji, Rebeca Rivera, Ramakrishna Sompallae, Nalini K Vudattu, Sven E Hoffner, Alimuddin Zumla, Lelany Pineda-Garcia, …

BMC infectious diseases, Vol.13(1), pp.125-125

03/06/2013

DOI: 10.1186/1471-2334-13-125

PMCID: PMC3599548

PMID: 23497342

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Abstract

Background A better understanding of the quality of cellular immune responses directed against molecularly defined targets will guide the development of TB diagnostics and identification of molecularly defined, clinically relevant M.tb vaccine candidates. Methods Recombinant proteins (n = 8) and peptide pools (n = 14) from M. tuberculosis (M.tb) targets were used to compare cellular immune responses defined by IFN-γ and IL-17 production using a Whole Blood Assay (WBA) in a cohort of 148 individuals, i.e. patients with TB + (n = 38), TB- individuals with other pulmonary diseases (n = 81) and individuals exposed to TB without evidence of clinical TB (health care workers, n = 29). Results M.tb antigens Rv2958c (glycosyltransferase), Rv2962c (mycolyltransferase), Rv1886c (Ag85B), Rv3804c (Ag85A), and the PPE family member Rv3347c were frequently recognized, defined by IFN-γ production, in blood from healthy individuals exposed to M.tb (health care workers). A different recognition pattern was found for IL-17 production in blood from M.tb exposed individuals responding to TB10.4 (Rv0288), Ag85B (Rv1886c) and the PPE family members Rv0978c and Rv1917c. Conclusions The pattern of immune target recognition is different in regard to IFN-γ and IL-17 production to defined molecular M.tb targets in PBMCs from individuals frequently exposed to M.tb. The data represent the first mapping of cellular immune responses against M.tb targets in TB patients from Honduras.

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Title: Subtitle: Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras
Creators: Nancy Alvarez-Corrales - National Autonomous University of Honduras
Raija K Ahmed - Swedish Institute
Carol A Rodriguez - National Autonomous University of Honduras
Kithiganahalli N Balaji - Indian Institute of Science Bangalore
Rebeca Rivera - National Autonomous University of Honduras
Ramakrishna Sompallae - University of Iowa
Nalini K Vudattu - Yale University
Sven E Hoffner - Swedish Institute
Alimuddin Zumla - University College London
Lelany Pineda-Garcia - National Autonomous University of Honduras
Markus Maeurer - Karolinska University Hospital
Resource Type: Journal article
Publication Details: BMC infectious diseases, Vol.13(1), pp.125-125
DOI: 10.1186/1471-2334-13-125
PMID: 23497342
PMCID: PMC3599548
NLM abbreviation: BMC Infect Dis
ISSN: 1471-2334
eISSN: 1471-2334
Publisher: BioMed Central
Language: English
Date published: 03/06/2013
Academic Unit: Pathology
Record Identifier: 9984622052002771

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