Differential effects of Francisella tularensis lipopolysaccharide on B lymphocytes

Riad M Rahhal; Tony J Vanden Bush; Molly K McLendon; Michael A Apicella; Gail A Bishop

doi:10.1189/jlb.1206765

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Differential effects of Francisella tularensis lipopolysaccharide on B lymphocytes

Journal article

Peer reviewed

Differential effects of Francisella tularensis lipopolysaccharide on B lymphocytes

Riad M Rahhal, Tony J Vanden Bush, Molly K McLendon, Michael A Apicella and Gail A Bishop

Journal of leukocyte biology, Vol.82(4), pp.813-820

10/2007

DOI: 10.1189/jlb.1206765

PMID: 17634278

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Abstract

Francisella tularensis, a designated Category A biological agent, can cause severe infection in humans. Previous studies have demonstrated a significant immunoprotective role for B lymphocytes in animal models, but the responses of human B lymphocytes to F. tularensis components are largely unknown. The LPS of F. tularensis is atypical and has been reported to lack biological activity on myeloid cells and mouse B cells. Our study characterized the immunological effects of highly purified LPS from different stains of F. tularensis on human B lymphocytes and compared these effects with those on mouse B cells and human monocyte-derived macrophages. Results indicate that marked differences exist between cell type and species in specific responses to this interesting bacterial component. In sharp contrast to responses of mouse splenic B cells or human macrophages, human peripheral B cells showed reproducibly elevated IL-6, TNF-alpha, and antibody production in response to F. tularensis LPS. Data also indicated that these activated human B lymphocytes may subsequently promote the activation of other immune cell types by direct cell-cell interaction. Further investigation into the potential usefulness of F. tularensis LPS as an adjuvant component of a more optimal subunit vaccine is warranted, as it is now clear that it is not biologically inactive, as assumed previously.

Cell Line

B-Lymphocytes - cytology

Adjuvants, Immunologic - pharmacology

Species Specificity

Vaccines, Subunit - chemistry

Humans

Vaccines, Subunit - immunology

Macrophages - cytology

Biological Warfare

Francisella tularensis - chemistry

Lymphocyte Activation - immunology

Models, Immunological

Animals

B-Lymphocytes - immunology

Adjuvants, Immunologic - chemistry

Lymphocyte Activation - drug effects

Antibody Formation - drug effects

Interleukin-6 - immunology

Lipopolysaccharides - pharmacology

Tumor Necrosis Factor-alpha - immunology

Lipopolysaccharides - chemistry

Mice

Macrophages - immunology

Details

Title: Subtitle: Differential effects of Francisella tularensis lipopolysaccharide on B lymphocytes
Creators: Riad M Rahhal - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Tony J Vanden Bush
Molly K McLendon
Michael A Apicella
Gail A Bishop
Resource Type: Journal article
Publication Details: Journal of leukocyte biology, Vol.82(4), pp.813-820
Publisher: United States
DOI: 10.1189/jlb.1206765
PMID: 17634278
ISSN: 0741-5400
eISSN: 1938-3673
Grant note: AI49993 / NIAID NIH HHS AI28847 / NIAID NIH HHS CA099997 / NCI NIH HHS AI044642 / NIAID NIH HHS
Language: English
Date published: 10/2007
Academic Unit: Microbiology and Immunology; President; Stead Family Department of Pediatrics; Gastroenterology, Hepatology, Pancreatology, and Nutrition; Internal Medicine
Record Identifier: 9984001207002771

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