Differential effects of N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists on spinal release of amino acids after development of acute arthritis in rats

K A Sluka; H H Jordan; W D Willis; K N Westlund

doi:10.1016/0006-8993(94)91956-9

Back

Differential effects of N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists on spinal release of amino acids after development of acute arthritis in rats

Journal article

Peer reviewed

Differential effects of N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists on spinal release of amino acids after development of acute arthritis in rats

K A Sluka, H H Jordan, W D Willis and K N Westlund

Brain research, Vol.664(1-2), pp.77-84

11/21/1994

DOI: 10.1016/0006-8993(94)91956-9

PMID: 7895049

View Online

Abstract

Following induction of acute knee joint arthritis in rats, an increase in the release of amino acids in the spinal dorsal horn occurs in two phases: (1) at the time of injection for all amino acids tested; and (2) a late prolonged phase for aspartate (Asp) and glutamate (Glu) (3.5-8 h). In the present study, the increased late phase release of Glu was reversed by posttreatment of the spinal cord with the N-methyl-D-aspartate (NMDA) receptor antagonist, AP7, but not with the non-NMDA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Asp late phase release in arthritic animals was unaffected by posttreatment of the spinal cord with either AP7 or CNQX. Arthritic animals became hyperalgesic to radiant heat stimuli by 4 h and this hyperalgesia was reversed by both CNQX and AP7. During the paw withdrawal latency (PWL) test for heat hyperalgesia, there was an increase in the glycine (Gly) and serine (Ser) concentrations in the dorsal horn. This increase in Gly and Ser was blocked by both CNQX and AP7. Indications of inflammation in arthritic animals posttreated with AP7, including increased joint circumference and temperature, were similar to animals that did not receive antagonists. Arthritic animals posttreated with CNQX, however, showed a reduction in the degree of joint swelling. Thus, both non-NMDA and NMDA receptors appear to play a role in the processing of the information evoked by stimuli in the periphery. The arthritis-induced release of Gly and Ser during the PWL test for heat hyperalgesia appears to be dependent on activation of both non-NMDA and NMDA receptors.

Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors

Acute Disease

Spinal Cord - metabolism

Arthritis - metabolism

Joints - drug effects

Rats

Male

Rats, Sprague-Dawley

Amino Acids - pharmacology

Amino Acids - metabolism

Arthritis - chemically induced

Animals

Microdialysis

Analysis of Variance

Reaction Time - drug effects

2-Amino-5-phosphonovalerate - analogs & derivatives

6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology

Excitatory Amino Acids - metabolism

Pain Measurement

Details

Title: Subtitle: Differential effects of N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists on spinal release of amino acids after development of acute arthritis in rats
Creators: K A Sluka - Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77555-0843
H H Jordan
W D Willis
K N Westlund
Resource Type: Journal article
Publication Details: Brain research, Vol.664(1-2), pp.77-84
Publisher: Netherlands
DOI: 10.1016/0006-8993(94)91956-9
PMID: 7895049
ISSN: 0006-8993
eISSN: 1872-6240
Grant note: NS 28064 / NINDS NIH HHS NS 01445 / NINDS NIH HHS NS 11255 / NINDS NIH HHS
Language: English
Date published: 11/21/1994
Academic Unit: Iowa Neuroscience Institute; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
Record Identifier: 9984040592902771

Metrics

15 Record Views

52 Times Cited - Web of Science

See more details