Differential effects of cerebellar inactivation on eyeblink conditioned excitation and inhibition

John H Freeman Jr; Hunter E Halverson; Amy Poremba

doi:10.1523/JNEUROSCI.4534-04.2005

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Differential effects of cerebellar inactivation on eyeblink conditioned excitation and inhibition

Journal article

Open access

Peer reviewed

Differential effects of cerebellar inactivation on eyeblink conditioned excitation and inhibition

John H Freeman Jr, Hunter E Halverson and Amy Poremba

The Journal of neuroscience, Vol.25(4), pp.889-895

01/26/2005

DOI: 10.1523/JNEUROSCI.4534-04.2005

PMCID: PMC1249522

PMID: 15673669

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https://doi.org/10.1523/JNEUROSCI.4534-04.2005View

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Abstract

The neural mechanisms underlying excitatory and inhibitory eyeblink conditioning were compared using muscimol inactivation of the cerebellum. In experiment 1, rats were given saline or muscimol infusions into the anterior interpositus nucleus ipsilateral to the conditioned eye before each of four daily excitatory conditioning sessions. Postinfusion testing continued for four more excitatory conditioning sessions. All rats were given a final test session after muscimol infusions. The muscimol infusions inactivated the cerebellar nuclei, lateral anterior lobe, crus I, rostral crus II, and lobule HVI ipsilateral to the conditioned eye. Acquisition of excitatory conditioning was completely prevented by muscimol inactivation. In experiment 2, there were four experimental phases. Phase 1 consisted of excitatory conditioning. In phase 2, rats were given saline or muscimol infusions before conditioned inhibition training. Phase 3 consisted of continued conditioned inhibition training with no drug infusions. In phase 4, all rats received a retardation test in which the inhibitory stimulus was paired with the unconditioned stimulus. Muscimol infusions blocked the expression of conditioned responses during phase 2. However, robust conditioned inhibition was evident in phases 3 and 4. The findings indicate that conditioned excitation and inhibition depend on different mechanisms.

Electromyography

Muscimol - pharmacology

Rats, Long-Evans

Cerebellum - drug effects

Infusions, Parenteral

Rats

Cerebellar Nuclei

Male

Functional Laterality

Cerebellum - physiology

Brain Stem - physiology

Conditioning, Classical - physiology

Brain Stem - drug effects

Deoxyglucose

Blinking - physiology

Autoradiography

Neural Inhibition - physiology

Animals

Muscimol - administration & dosage

Details

Title: Subtitle: Differential effects of cerebellar inactivation on eyeblink conditioned excitation and inhibition
Creators: John H Freeman Jr - Department of Psychology, University of Iowa, Iowa City, Iowa 52242, USA. john-freeman@uiowa.edu
Hunter E Halverson
Amy Poremba
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.25(4), pp.889-895
Publisher: United States
DOI: 10.1523/JNEUROSCI.4534-04.2005
PMID: 15673669
PMCID: PMC1249522
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: MH065483 / NIMH NIH HHS R01 MH065483 / NIMH NIH HHS
Language: English
Date published: 01/26/2005
Academic Unit: Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute
Record Identifier: 9984065752802771

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