Journal article
Differential effects of nitroblue tetrazolium on the hemodynamic responses elicited by activation of α 1-adrenoceptors and 5-HT 2 receptors in conscious rats
European journal of pharmacology, Vol.535(1), pp.248-252
2006
DOI: 10.1016/j.ejphar.2006.01.060
Abstract
This study determined the effects of the lipophobic electron acceptor, nitroblue tetrazolium (5
μmol/kg, i.v.) on the vasoconstrictor responses elicited by the 5-hydroxytryptamine
2 (5-HT
2) receptor agonist, α-methyl-5-HT (5–50
μg/kg, i.v.) and the α
1-adrenoceptor agonist, phenylephrine (2.5–20
μg/kg, i.v.) in conscious Sprague–Dawley rats. The systemic injection of nitroblue tetrazolium elicited pronounced hemodynamic responses that subsided by 10–15
min. Prior to the administration of nitroblue tetrazolium, the injections of α-methyl-5-HT and phenylephrine elicited dose-dependent increases in mean arterial blood pressure and mesenteric, renal and hindquarter vascular resistances. After administration of nitroblue tetrazolium, the vasoconstrictor responses elicited by α-methyl-5-HT were augmented whereas those elicited by phenylephrine were diminished. These results are consistent with the possibility that nitroblue tetrazolium interacts with the extracellular ligand-binding domains of 5-HT
2 receptors and α
1-adrenoceptor and that this interaction has opposite effects on activities of these G protein coupled receptors.
Details
- Title: Subtitle
- Differential effects of nitroblue tetrazolium on the hemodynamic responses elicited by activation of α 1-adrenoceptors and 5-HT 2 receptors in conscious rats
- Creators
- Joy R. Owen - Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7389, USAJames N. Bates - University of Iowa, AnesthesiaStephen J. Lewis - Case Western Reserve University
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.535(1), pp.248-252
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.ejphar.2006.01.060
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Language
- English
- Date published
- 2006
- Academic Unit
- Anesthesia
- Record Identifier
- 9984424843902771
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