Journal article
Differential effects of ouabain on the vasodilator actions of nitric oxide and S-nitrosothiols in vivo: Relevance to the identity of EDRF/EDHF
Vascular pharmacology, Vol.45(6), pp.383-394
2006
DOI: 10.1016/j.vph.2006.06.008
PMID: 16861050
Abstract
This study examined the role of Na
+/K
+-ATPase in the vasodilator actions of nitric oxide (NO),
S-nitrosothiols and the endothelium-dependent agonist, acetylcholine.
The vasodilator responses elicited by intravenous injections of (i) the NO-donors, sodium nitroprusside and MAHMA NONOate, (ii) the
S-nitrosothiols,
l-
S-nitrosocysteine and
S-nitrosocoenzyme A, and (iii) acetylcholine, in urethane-anesthetized rats.
The NO-donors,
S-nitrosothiols and acetylcholine elicited dose-dependent depressor responses and reductions in hindquarter (HQR) and mesenteric (MR) vascular resistances. The depressor responses and associated reductions in HQR elicited by NO-donors were markedly attenuated after injection of ouabain. In contrast, the depressor responses and reductions in HQR elicited by the
S-nitrosothiols and acetylcholine were not affected. The reductions in MR elicited by all vasodilator agents were exaggerated after injection of ouabain. Finally, the decomposition of sodium nitroprusside, MAHMA NONOate,
l-
S-nitrosocysteine and
S-nitrosocoenzyme A to NO upon addition to rat blood or vascular preparations was not affected by ouabain.
This study demonstrates that ouabain has opposing effects on NO-mediated vasodilation in resistance arteries in the hindquarter and mesenteric beds of the rat. The similarity of effects of ouabain on the vasodilator actions of acetylcholine,
l-
S-nitrosocysteine and
S-nitrosocoenzyme A as opposed to the NO-donors supports the possibility that endothelium-derived relaxing factor released by acetylcholine in resistance arteries is an
S-nitrosothiol.
Details
- Title: Subtitle
- Differential effects of ouabain on the vasodilator actions of nitric oxide and S-nitrosothiols in vivo: Relevance to the identity of EDRF/EDHF
- Creators
- Stephen J Lewis - Department of Physiology and Pharmacology, University of Georgia, Athens, GA, USAMark D Travis - Department of Pharmacology, University of Iowa, Iowa City, IA, USAMaleka P Hashmi-Hill - Department of Physiology and Pharmacology, University of Georgia, Athens, GA, USAKevin Sandock - Department of Physiology and Pharmacology, University of Georgia, Athens, GA, USATom P Robertson - Department of Physiology and Pharmacology, University of Georgia, Athens, GA, USAJames N Bates - Department of Anesthesia, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Vascular pharmacology, Vol.45(6), pp.383-394
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.vph.2006.06.008
- PMID
- 16861050
- ISSN
- 1537-1891
- eISSN
- 1879-3649
- Language
- English
- Date published
- 2006
- Academic Unit
- Anesthesia
- Record Identifier
- 9984006348402771
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