Journal article
Differential effects of peroxynitrite on the function of arginine vasopressin V 1a receptors and alpha 1-adrenoceptors in vivo
Vascular pharmacology, Vol.46(1), pp.24-34
2007
DOI: 10.1016/j.vph.2006.06.004
PMID: 16861049
Abstract
The aim of this study was to provide evidence that peroxynitrite may differentially affect the function of arginine vasopressin (AVP) V
1a receptors and α
1-adrenoceptors in vascular smooth muscle of the rat
The vasoconstrictor responses elicited by AVP, or the α
1-adrenoceptor agonist, phenylephrine, were determined in anesthetized rats before and after injections of (i) peroxynitrite, the thiol chelator,
para-hydroxymercurobenzoic acid (PHMBA), or the electron acceptor, nitroblue tetrazolium (NBT). The ability of the reducing agent, glutathione, to reverse the loss of response to phenylephrine and AVP in peroxynitrite-treated rats was also examined.
The AVP-induced responses were suppressed 10–20 min but not 60–70 min after the administration of peroxynitrite. Glutathione reversed the above loss of response to AVP at 10–20 min. The responses elicited by phenylephrine were suppressed 10–20 min and 60–70 min after administration of peroxynitrite. Glutathione did not reverse the above losses of response to phenylephrine. In addition, the vasoconstrictor actions of AVP and phenylephrine were markedly suppressed after administration of PHMBA or nitroblue tetrazolium.
The above findings provide evidence that exogenously administered peroxynitrite may differentially affect the function of AVP V
1a receptors and α
1-adrenoceptors in vascular smooth muscle of the rat. The possibility that peroxynitrite impairs AVP V
1a receptor function by transient oxidation events whereas peroxynitrite impairs α
1-adrenoceptor function by transient oxidation and permanent nitration events will be discussed.
Details
- Title: Subtitle
- Differential effects of peroxynitrite on the function of arginine vasopressin V 1a receptors and alpha 1-adrenoceptors in vivo
- Creators
- Stephen J Lewis - Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602-7389, USAAzizul Hoque - Department of Cardiology, The University of Louisville, Louisville, Kentucky, USAKevin Sandock - Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602-7389, USATom P Robertson - Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602-7389, USAJames N Bates - Department of Anesthesia, University of Iowa, Iowa City, Iowa, USANeil W Kooy - Department of Pediatrics, Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio, USA
- Resource Type
- Journal article
- Publication Details
- Vascular pharmacology, Vol.46(1), pp.24-34
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.vph.2006.06.004
- PMID
- 16861049
- ISSN
- 1537-1891
- eISSN
- 1879-3649
- Language
- English
- Date published
- 2007
- Academic Unit
- Anesthesia
- Record Identifier
- 9984006349702771
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