Journal article
Differential expression of CD22 (Lyb8) on murine B cells
International immunology, Vol.8(7), pp.1121-1129
07/1996
DOI: 10.1093/intimm/8.7.1121
PMCID: PMC2859887
PMID: 8757957
Abstract
Previous studies have established the distribution, biochemistry and functional attributes of human CD22, a B cell-restricted glycoprotein. Recently, molecular cloning of the murine CD22 equivalent revealed this molecule to be the same as the previously described Lyb8 alloantigen. Using the antl-Lyb8 mAb Cy34.1.2, the present report documents the expression patterns of CD22 within the murine B cell compartment. The results demonstrate that in the bone marrow, murine CD22 is absent on the surface of pro-B cells, pre-B cells and newly emerging lgM
+
B cells. CD22 is present at a low density on immature IgM
hi
B cells and fully expressed on mature recirculatlng B cells. In the periphery, murine CD22 is expressed at mature levels on all B cell subsets Including follicular, marginal zone, B1 and switched B cells. Further studies showed CD22 to be retained on activated murine B cells for extended periods. Finally, In combination with CD23 and heat stable antigen, CD22 can be used to delineate the immature splenic B cells, and distinguish them from follicular and marginal zone cells. Together, the results demonstrate murine CD22 to be a useful pan marker for all mature B cell subsets.
Details
- Title: Subtitle
- Differential expression of CD22 (Lyb8) on murine B cells
- Creators
- Loren D Erickson - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USALorraine T Tygrett - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USASudershan K Bhatia - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USAKenneth H Grabstein - Corixa Corp., Seattle, WA 98101, USAThomas J Waldschmidt - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- International immunology, Vol.8(7), pp.1121-1129
- DOI
- 10.1093/intimm/8.7.1121
- PMID
- 8757957
- PMCID
- PMC2859887
- ISSN
- 0953-8178
- eISSN
- 1460-2377
- Language
- English
- Date published
- 07/1996
- Academic Unit
- Pathology; Radiation Oncology
- Record Identifier
- 9984047687102771
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