Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus

Srihari B Janardhanam; Sivaraman Prakasam; Venkatesh T Swaminathan; Krithika N Kodumudi; Susan L Zunt; Mythily Srinivasan

doi:10.1016/j.archoralbio.2011.10.013

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Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus

Journal article

Peer reviewed

Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus

Srihari B Janardhanam, Sivaraman Prakasam, Venkatesh T Swaminathan, Krithika N Kodumudi, Susan L Zunt and Mythily Srinivasan

Archives of oral biology, Vol.57(5), pp.495-502

05/01/2012

DOI: 10.1016/j.archoralbio.2011.10.013

PMID: 22119043

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Abstract

Oral lichen planus (OLP) is a chronic inflammatory condition of the mucosa mediated by a complex signalling network between the keratinocytes and the sub-epithelial lymphocytes. Since OLP occurs in constantly renewing epithelium continuously exposed to commensals, we hypothesised that the epithelial cell microflora interactions may mediate the persistent inflammation. By virtue of their ability to respond to most oral commensal microorganisms, the toll like receptor-2 (TLR-2) and TLR-4 are the most widely investigated receptors in oral diseases. The overall objective of this study was to investigate the role of TLR-2 and TLR-4 in OLP. Systemically healthy OLP and control subjects were recruited after obtaining the institutional review board approval. Expression of TLR-2 and TLR-4 proteins and transcripts in the tissue epithelium and in the epithelial cells isolated from saliva were determined by immunohistochemistry and quantitative real-time polymerase chain reaction respectively. The tissue epithelium and the salivary epithelial cells expressed reduced TLR-2 and increased TLR-4 proteins and transcripts in OLP. The salivary epithelial cells from OLP subjects secreted elevated IL-12. However, upon stimulation with bacterial lipopolysaccharide the epithelial cells from OLP exhibited a mixed Th1 (IL-12) and Th2 (IL-4) response. Presence of dexamethasone significantly reduced inflammatory cytokines in the in vitro stimulated cultures of salivary epithelial cells from OLP subjects. Collectively, our data support a critical role for the host-microbial interactions in the OLP pathogenesis. The potential use of exfoliated oral epithelial cells in saliva for functional analysis exponentially increases its value as biological specimen for clinical research.

Biomarkers - metabolism

Case-Control Studies

Dexamethasone - pharmacology

Enzyme-Linked Immunosorbent Assay

Epithelial Cells - metabolism

Female

Humans

Immunohistochemistry

Interleukin-12 - metabolism

Lichen Planus, Oral - metabolism

Lipopolysaccharides - pharmacology

Male

Middle Aged

Photomicrography

Real-Time Polymerase Chain Reaction

Saliva - cytology

Toll-Like Receptor 2 - metabolism

Toll-Like Receptor 4 - metabolism

Details

Title: Subtitle: Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus
Creators: Srihari B Janardhanam - Indiana University
Sivaraman Prakasam - Indiana University
Venkatesh T Swaminathan - Indiana University
Krithika N Kodumudi - Indiana University
Susan L Zunt - Indiana University
Mythily Srinivasan - Indiana University
Resource Type: Journal article
Publication Details: Archives of oral biology, Vol.57(5), pp.495-502
DOI: 10.1016/j.archoralbio.2011.10.013
PMID: 22119043
ISSN: 0003-9969
eISSN: 1879-1506
Language: English
Date published: 05/01/2012
Academic Unit: Periodontics
Record Identifier: 9984446421602771

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