Journal article
Differential expression of ovine innate immune genes by preterm and neonatal lung epithelia infected with respiratory syncytial virus
Viral immunology, Vol.19(2), pp.316-323
2006
DOI: 10.1089/vim.2006.19.316
PMCID: PMC2791066
PMID: 16817774
Abstract
Preterm infants have increased susceptibility to severe manifestations of respiratory syncytial virus (RSV) infection. The cause(s) for this age-dependent vulnerability is/are not well-defined, but alterations in innate immune products have been implicated. In sheep, RSV disease severity has similar age-dependent characteristics and sheep have several related innate molecules for study during pulmonary infection including surfactant protein A (SP-A), surfactant protein D (SP-D), sheep beta defensin 1 (SBD1), monocyte chemotactic protein 1 (MCP1), and Toll-like receptor 4 (TLR4). However, the in vivo cellular gene expression as a response to RSV infection is poorly understood. In this study, the effect of RSV infection on expression of these innate immune genes was determined for bovine RSV-infected (bRSV+ fluorescence) epithelial cells, adjacent cells lacking bRSV antigen (adjoining cells lacking fluorescence), and control cells from non-infected lung using laser capture microdissection (LCM) and real-time RT-PCR. Control lambs had increased expression of innate immune molecules in full term (term) compared to preterm epithelia with statistical significance in SBD1, SP-D, and TLR4 mRNA. Infected cells (bRSV+ fluorescent cells) had consistently higher mRNA levels of SP-A (preterm and term), MCP1 (preterm and term), and SP-D (preterm). Interestingly, bRSV- cells of infected term lambs had significantly reduced SP-D mRNA expression compared to bRSV+ and control epithelia, suggesting that RSV infected cells may regulate the adjacent epithelial SP-D expression. This study defines specific innate immune components (e.g., SBD1, SP-D, and TLR4) that have differential age-dependent expression in the airway epithelia. Furthermore, cellular bRSV infection enhanced certain innate immune components while suppressing adjacent cellular SP-D expression in term animals. These in vivo gene expression results provide a framework for future studies on age-dependent susceptibility to RSV and RSV pathogenesis.
Details
- Title: Subtitle
- Differential expression of ovine innate immune genes by preterm and neonatal lung epithelia infected with respiratory syncytial virus
- Creators
- Kenji Kawashima - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University Ames, Iowa 50011-1250, USADavid K MeyerholzJack M GallupBranka GruborTatjana LazicHoward D LehmkuhlMark R Ackermann
- Resource Type
- Journal article
- Publication Details
- Viral immunology, Vol.19(2), pp.316-323
- DOI
- 10.1089/vim.2006.19.316
- PMID
- 16817774
- PMCID
- PMC2791066
- NLM abbreviation
- Viral Immunol
- ISSN
- 0882-8245
- eISSN
- 1557-8976
- Grant note
- R01 AI062787 / NIAID NIH HHS 05R01AI062787-02 / NIAID NIH HHS R01 AI062787-04 / NIAID NIH HHS K08 AI055499 / NIAID NIH HHS 5K08AI055499-03 / NIAID NIH HHS
- Language
- English
- Date published
- 2006
- Academic Unit
- Pathology
- Record Identifier
- 9984083247702771
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