Journal article
Differential expression of sheep beta-defensin-1 and -2 and interleukin 8 during acute Mannheimia haemolytica pneumonia
Microbial pathogenesis, Vol.37(1), pp.21-27
2004
DOI: 10.1016/j.micpath.2004.04.003
PMID: 15194156
Abstract
Beta-defensins are antimicrobial peptides produced by several cell types, including respiratory epithelia and leukocytes. Expression of some beta-defensins is increased by bacterial-induced inflammatory responses whereas expression of other beta-defensins is constitutive. Two beta-defensins are expressed in lungs of sheep (sheep beta-defensin-1 and -2; SBD-1/-2) and expression of SBD-1 is increased during parainfluenza virus type 3 (PI-3) infection. The effect of
Mannheimia haemolytica, a Gram-negative bacteria known to induce expression of bovine beta-defensins and NF-kappa B in lung, has not been determined for SBD-1/-2. In this study, different concentrations of
M. haemolytica were inoculated into pulmonary bronchi of lambs. SBD-1 and SBD-2 mRNA levels detected by real time reverse transcriptase polymerase chain reaction in lung homogenates did not increase. In fact, SBD-1 mRNA levels were significantly decreased with the highest administered inoculum concentration (10
9). In contrast, mRNA levels of interleukin-8 (IL-8) were significantly increased over controls and progressively increased with
M. haemolytica concentrations. Co-inoculation of
M. haemolytica with xylitol, an osmotic agent, did not alter mRNA levels of SBD-1, SBD-2 or IL-8. SBD-1 mRNA expression was detected in lung epithelia, but not in leukocytes. This study suggests that SDB-1 expression occurs in epithelia and decreases during severe bacterial pneumonia, which is in contrast to the increase that occurs with PI-3 infection.
Details
- Title: Subtitle
- Differential expression of sheep beta-defensin-1 and -2 and interleukin 8 during acute Mannheimia haemolytica pneumonia
- Creators
- Mark R Ackermann - Department of Veterinary Pathology, College of Veterinary Medicine, 2738 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USAJack M Gallup - Department of Veterinary Pathology, College of Veterinary Medicine, 2738 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USAJoseph Zabner - Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USARichard B Evans - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USACharles W Brockus - Department of Veterinary Pathology, College of Veterinary Medicine, 2738 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USADavid K Meyerholz - Department of Veterinary Pathology, College of Veterinary Medicine, 2738 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USABranka Grubor - Department of Veterinary Pathology, College of Veterinary Medicine, 2738 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USAKim A Brogden - Respiratory Diseases of Livestock Research Unit, USDA-Agriculture Research Service, National Animal Disease Center, Ames, IA 50010, USA 1 Present address: Department of periodontics and Dows Institute for Dental Research College of Dentistry University of Iowa, Iowa city, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Microbial pathogenesis, Vol.37(1), pp.21-27
- DOI
- 10.1016/j.micpath.2004.04.003
- PMID
- 15194156
- NLM abbreviation
- Microb Pathog
- ISSN
- 0882-4010
- eISSN
- 1096-1208
- Publisher
- Elsevier India Pvt Ltd
- Language
- English
- Date published
- 2004
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Pathology; Periodontics; Internal Medicine
- Record Identifier
- 9984083881902771
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