Journal article
Differential expression of the A and B isoforms of progesterone receptor in human endometrial cancer cells. Only progesterone receptor B is induced by estrogen and associated with strong transcriptional activation
Annals of the New York Academy of Sciences, Vol.828, p.17
09/26/1997
PMID: 9329820
Abstract
The synapsins are a family of synaptic vesicle-associated phosphoproteins thought to regulate the availability of vesicles for neurotransmitter release. In order to assess variability of synapsin isoform expression, we compared the localization of synapsins Ia, Ib, IIa, and IIb in the inner plexiform layer of the rat retina. Double labeling in conjunction with confocal fluorescence and electron microscopy allowed imaging of synapsin I and II immunoreactivity within single presynaptic terminals. No qualitative differences were observed between expression of the a and b isoforms of synapsin I in individual terminals; likewise, the a and b isoforms of synapsin II were identically distributed. In contrast, marked differences were seen upon comparison of synapsin I and synapsin II expression in single terminals. Our results indicate the existence of three classes of presumed amacrine cell synaptic terminals: synapsin I+/synapsin II-, synapsin I-/synapsin II+, and synapsin I+/synapsin II+. Each class of synapse has a different distribution among five IPL sublayers, suggesting that they represent different subpopulations of amacrine cells. Double labeling with an antibody to choline acetyltransferase indicates that synapsin I-/II+ terminals may be those of cholinergic amacrine cells. Furthermore, all synapsin II+ terminals appear to be distinct from those expressing the GABA synthetic enzyme glutamic acid decarboxylase. The observed variations in synapsin content suggest the existence of presynaptic terminal heterogeneity that is not apparent from conventional morphological studies.
Details
- Title: Subtitle
- Differential expression of the A and B isoforms of progesterone receptor in human endometrial cancer cells. Only progesterone receptor B is induced by estrogen and associated with strong transcriptional activation
- Creators
- Kimberly Kay Leslie - University of Iowa, Obstetrics and GynecologyNirmala S KumarJennifer RicherGareth OwenGlenn TakimotoKathryn B Horwitz - University of Iowa, Obstetrics and GynecologyCarol Lange
- Resource Type
- Journal article
- Publication Details
- Annals of the New York Academy of Sciences, Vol.828, p.17
- Publisher
- United States
- PMID
- 9329820
- ISSN
- 0077-8923
- eISSN
- 1749-6632
- Grant note
- 2P30CA46934-09 / NCI NIH HHS 5R29CA67313-02 / NCI NIH HHS DKO7446-55T32 / NIDDK NIH HHS
- Language
- English
- Date published
- 09/26/1997
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557307402771
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