Differential inhibition of cellular and viral pp60src kinase by P1, P4, Di(adenosine-5') tétraphosphate

Barcey T Levy; Laurie K Sorge; Cynthia C Drum; Patricia F Maness

doi:10.1128/mcb.3.10.1718-1723.1983

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Differential inhibition of cellular and viral pp60src kinase by P1, P4, Di(adenosine-5') tétraphosphate

Journal article

Peer reviewed

Differential inhibition of cellular and viral pp60src kinase by P1, P4, Di(adenosine-5') tétraphosphate

Barcey T Levy, Laurie K Sorge, Cynthia C Drum and Patricia F Maness

Molecular and cellular biology, Vol.3(10), pp.1718-1723

1983

DOI: 10.1128/mcb.3.10.1718-1723.1983

PMCID: PMC370032

PMID: 6196614

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Abstract

We contrasted the protein kinase activities of pp60v-src, the transforming protein of Rous sarcoma virus, and its normal cellular homolog pp60c-src with respect to inhibition by P1,P4-di(adenosine-5')tetraphosphate by using the immune complex protein kinase assay. The concentration of P1,P4-di(adenosine-5')tetraphosphate required for 50% inhibition of pp60v-src kinase (1 microM) was found to be significantly lower than that required for inhibition of pp60c-src kinase (46 microM). Viral and cellular pp60src kinases differed to a lesser extent with respect to inhibition by adenosine-5'-tetraphosphate, di(guanosine-5')tetraphosphate, and ADP. No significant differences were found in the ATP Km values of pp60v-src (0.108 +/- 0.048 microM) and pp60c-src kinases (0.056 +/- 0.012 microM). These results demonstrate that the protein kinase activities of viral and cellular pp60src are functionally distinguishable, particularly on the basis of enhanced sensitivity of the viral enzyme to inhibition by P1,P4-di(adenosine-5')tetraphosphate. These functional differences are likely to be due to differences in the conformation of the active site and may be important for determining transformation potential.

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains

Biological and medical sciences

Details

Title: Subtitle: Differential inhibition of cellular and viral pp60src kinase by P1, P4, Di(adenosine-5') tétraphosphate
Creators: Barcey T Levy - Univ. North Carolina, school medicine
Laurie K Sorge - Univ. North Carolina, school medicine
Cynthia C Drum - Univ. North Carolina, school medicine
Patricia F Maness - Univ. North Carolina, school medicine
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.3(10), pp.1718-1723
Publisher: American Society for Microbiology; Washington, DC
DOI: 10.1128/mcb.3.10.1718-1723.1983
PMID: 6196614
PMCID: PMC370032
ISSN: 0270-7306
eISSN: 1098-5549
Language: English
Date published: 1983
Academic Unit: Epidemiology; Family and Community Medicine
Record Identifier: 9984024563802771

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