Journal article
Differential modulation of hepatic cytochrome P‐450 enzymes in rat and syrian hamster by 4′‐trifluoromethyl‐2,3,4,5‐tetrachlorobiphenyl
Journal of biochemical toxicology, Vol.9(5), pp.241-248
10/1994
DOI: 10.1002/jbt.2570090504
PMID: 7853359
Abstract
The effects of a single injection (40 mg/kg) of 4′‐trifluoromethyl‐2,3,4,5‐tetrachlorobiphenyl (CF3) on hepatic cytochrome P‐450 monooxygenases were assessed in rat and syrian hamster. The CF3 treatment significantly increased the total amount of cytochrome P‐450 in both species. In rats, CF3 treatment caused marked increases in ethoxyresorufin O‐deethylase (EROD), arylhydrocarbon hydroxylase (AHH), and testosterone 7α‐hydroxylase activities but significantly reduced the activities of benzphetamine N‐demethylase (BzND), erythromycin N‐demethylase (ErND), testosterone 6β, 16α, and 16β‐hydroxylases, and formation of androstenedione. Administration of CF3 to hamsters strongly induced the activities of EROD, AHH, BzND, testosterone 15α, and 16α‐hydroxylases, and androstenedione production, whereas ErND, testosterone 6β, and 7α‐hydroxylases were decreased. Administration of CF3 to rats induced the CYP1A family proteins and CYP2A1, while CF3 reduced the level of CYP2B1, and, to a lesser extent, of CYP6β2. In hamsters, CF3 treatment significantly induced the CYP1A2, CYP2A1, CYP2A8, and CYP2B1 isozymes, whereas the CYP6β2 level was decreased. The ability of hepatic microsomes to activate aflatoxin B1 and benzo(a)pyrene was elevated by CF3 treatment in hamsters, while activation of aflatoxin B1 was decreased in microsomes from CF3‐treated rats. These results showed differences in the CF3‐induced pattern of rat and hamster cytochrome P‐450 monooxygenases.
Details
- Title: Subtitle
- Differential modulation of hepatic cytochrome P‐450 enzymes in rat and syrian hamster by 4′‐trifluoromethyl‐2,3,4,5‐tetrachlorobiphenyl
- Creators
- Marie‐Hélène BaniJean‐Franco̧is NarbonneMorio FukuharaFusao UshioLarry W Robertson
- Resource Type
- Journal article
- Publication Details
- Journal of biochemical toxicology, Vol.9(5), pp.241-248
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Weinheim
- DOI
- 10.1002/jbt.2570090504
- PMID
- 7853359
- ISSN
- 0887-2082
- eISSN
- 1522-7146
- Number of pages
- 8
- Grant note
- Ministère de la Recherche et de l'Environnement (France) Science and technology Agency of Japan (Japan) NATO Collaborative Research Grant (Belgium)
- Language
- English
- Date published
- 10/1994
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984001103702771
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