Differential role for stromal interacting molecule 1 in the regulation of vascular function

Modar Kassan; Wei Zhang; Karima Ait Aissa; Judith Stolwijk; Mohamed Trebak; Khalid Matrougui

doi:10.1007/s00424-014-1556-5

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Differential role for stromal interacting molecule 1 in the regulation of vascular function

Journal article

Peer reviewed

Differential role for stromal interacting molecule 1 in the regulation of vascular function

Modar Kassan, Wei Zhang, Karima Ait Aissa, Judith Stolwijk, Mohamed Trebak and Khalid Matrougui

Pflügers Archiv, Vol.467(6), pp.1195-1202

2015

DOI: 10.1007/s00424-014-1556-5

PMCID: PMC4843990

PMID: 24965067

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https://www.ncbi.nlm.nih.gov/pmc/articles/4843990View

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Abstract

We determined the in vivo role of stromal-interacting molecule 1 (STIM1) in the regulation of vascular function using endothelial cell (EC)- and smooth-muscle (SM)-specific knockout mice. Systolic blood pressure and glucose levels were similar in all mice (Stim1 SMC−/− , Stim1 SMC−/+ , Stim1 EC−/− , Stim1 EC−/+ ), but body weight was reduced in Stim1 EC−/− and Stim1 SMC−/− mice. The contraction of arteries in response to phenylephrine was significantly reduced in Stim1 SMC−/− mice only. However, contraction to thromboxane and KCl was similar in all groups. The endothelium-dependent relaxation (EDR) was impaired in Stim1 EC−/+ and drastically reduced in Stim1 EC−/− mice while the endothelium-independent vasorelaxation was similar among all groups. Acute downregulation of STIM1 in arteries reduced EDR and the contractile response to phenylephrine, while the contractile response to thromboxane was not affected. NADPH oxidase activity was increased only in Stim1 EC−/+ and Stim1 EC−/− mice. Calcium (Ca 2+ ) entry in endothelial cells stimulated with thrombin and histamine had the pharmacological features of store-operated Ca 2+ entry (SOCE) and was dependent on STIM1 expression. We conclude that STIM1 plays opposing roles in vascular smooth muscle vs. endothelial cells in the regulation of vascular reactivity.

Biomedical and Life Sciences

Biomedicine

Cell Biology

Human Physiology

Ion Channels

Molecular Medicine

Neurosciences

Receptors

Receptors and Transporters

Details

Title: Subtitle: Differential role for stromal interacting molecule 1 in the regulation of vascular function
Creators: Modar Kassan - Department of Physiological Sciences
Wei Zhang - SUNY Polytechnic Institute
Karima Ait Aissa - Department of Physiological Sciences
Judith Stolwijk - SUNY Polytechnic Institute
Mohamed Trebak - SUNY Polytechnic Institute
Khalid Matrougui - Department of Physiological Sciences
Resource Type: Journal article
Publication Details: Pflügers Archiv, Vol.467(6), pp.1195-1202
DOI: 10.1007/s00424-014-1556-5
PMID: 24965067
PMCID: PMC4843990
NLM abbreviation: Pflugers Arch
ISSN: 0031-6768
eISSN: 1432-2013
Publisher: Springer Berlin Heidelberg
Language: English
Date published: 2015
Academic Unit: Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984359908502771

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