Journal article
Differential screening and suppression subtractive hybridization identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line
Nucleic acids research, Vol.26(4), pp.1116-1123
02/15/1998
DOI: 10.1093/nar/26.4.1116
PMCID: PMC147366
PMID: 9461476
Abstract
Differences in gene expression are likely to explain the phenotypic differences between hormone-responsive and hormone-unresponsive breast cancer. We have identified differentially expressed cDNAs in the estrogen receptor (ER)-positive MCF7 breast carcinoma cell line compared with the ER-negative MDA-MB-231 breast carcinoma cell line. Differential screening isolated four differentially expressed genes: cytokeratin 8, cytokeratin 18, Hsp27 and GPCR -Br. To identify differentially expressed genes of lower abundance, suppression subtractive hybridization was utilized and 29 differentially expressed clones were isolated. Sequence analysis revealed that 11 clones were from previously described genes: HEK8, neuropeptide Y receptor Y1, p21 WAF-1, p55 PIK, cytokeratin 18 (cloned twice), fructose-1,6-biphosphatase, cytokeratin 8, TGFbeta1 binding protein, elongation factor 1alpha2 and pS2. The remaining 18 clones did not match sequences in the GenBank/EMBL database, indicating that they may be novel genes. Expression of pS2, neuropeptide Y receptor Y1 and three novel clones was induced by estradiol, indicating estrogen-responsiveness. The expression pattern of one novel gene, DEME -6, correlated with expression of ER and ERF -1/ AP -2gamma in a panel of breast carcinoma cell lines. A 2.6 kb cDNA of DEME -6 was sequenced and contains an open reading frame of 574 amino acids that demonstrates 62.4% similarity with a gene from Caenorhabditis elegans chromosome III. Expression of DEME -6 was also detected in primary breast carcinomas but not in normal breast tissue, as determined by RT-PCR. These findings support the hypothesis that a set of genes coordinately regulated with ER , but not necessarily estradiol-responsive, are characteristic of the hormone-responsive breast cancer phenotype.
Details
- Title: Subtitle
- Differential screening and suppression subtractive hybridization identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line
- Creators
- Wayne W Kuang - Department of Surgery, Stanford University, Stanford, CA 94305, USADevon A Thompson - Department of Surgery, Stanford University, Stanford, CA 94305, USAR V Hoch - Department of Surgery, Stanford University, Stanford, CA 94305, USARonald J Weigel - Department of Surgery, Stanford University, Stanford, CA 94305, USA
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.26(4), pp.1116-1123
- DOI
- 10.1093/nar/26.4.1116
- PMID
- 9461476
- PMCID
- PMC147366
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Language
- English
- Date published
- 02/15/1998
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984024556002771
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