Journal article
Differential structure-function requirements of the transmembranal domain of the B cell antigen receptor
The Journal of experimental medicine, Vol.176(4), pp.1025-1031
10/01/1992
DOI: 10.1084/jem.176.4.1025
PMCID: PMC2119374
PMID: 1402648
Abstract
By generating phosphorylcholine (PC)-specific, wild-type (mu), and chimeric (mu-I-A alpha) antigen receptor transfectants of mature B cells, we have shown that the COOH terminus of the mu heavy chain is essential for three major functions: immediate signal transduction (measured as changes in intracellular Ca2+), antigen presentation, and induction of immunoglobulin M secretion. A more detailed analysis of structural requirements of the COOH-terminal domains contributing to these functions was achieved by systematically replacing the spacer, cytoplasmic, and transmembranal domains of the mu-I-A alpha chimeric chain with those of mu. Using this rescue approach, we show that the carboxyl two-thirds of the transmembranal domain (proximal to the cytoplasmic domain) is required for induction of intracellular Ca2+, whereas the complete transmembranal domain is required for the function of antigen presentation but is dispensable for induction of antibody secretion.
Details
- Title: Subtitle
- Differential structure-function requirements of the transmembranal domain of the B cell antigen receptor
- Creators
- V S Parikh - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235G A Bishop - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235K J Liu - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235B T Do - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235M R Ghosh - The University of Texas Southwestern Medical CenterB S Kim - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235P W Tucker - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235
- Resource Type
- Journal article
- Publication Details
- The Journal of experimental medicine, Vol.176(4), pp.1025-1031
- DOI
- 10.1084/jem.176.4.1025
- PMID
- 1402648
- PMCID
- PMC2119374
- ISSN
- 0022-1007
- eISSN
- 1540-9538
- Language
- English
- Date published
- 10/01/1992
- Academic Unit
- Microbiology and Immunology; President
- Record Identifier
- 9984002394002771
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