Differential transition metal uptake and fluorescent probe localization in hippocampal slices

Alan R Kay; Sean M Rumschik

doi:10.1039/c1mt00024a

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Journal article

Differential transition metal uptake and fluorescent probe localization in hippocampal slices

Alan R Kay and Sean M Rumschik

Metallomics, Vol.3(8), pp.829-837

08/2011

DOI: 10.1039/c1mt00024a

PMID: 21681308

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Abstract

Metals are taken up by the combined action of metal transporters and ion channels. In this communication we have measured the uptake of the biologically important transition metals Mn, Fe, Co, Ni, Cu, Zn and Cd by rat and mouse hippocampal slices using the fluorescent probes FluoZin-3 (FZ3) and Newport Green (NPG), introduced by acetoxymethyl ester (AM) loading. The combination of metals and probes is also used to attempt to localize cellular sites into which metals translocate. We show that FZ3 and NPG partition into different cellular compartments; FZ3 into neuropil, whereas NPG localizes in neuropil and compartments within the cell bodies of neurons. Ni, Zn and Cd pass across the plasma membrane and then accumulate in intracellular vesicles and within intracellular membranes of cell bodies. The latter accumulate Cd, while synaptic vesicles take up Co. The passage of Mn, Cu and Fe into cells can be detected but there is some uncertainty about their disposition within the cell. All of our experiments are consistent with metals accumulating in intracellular compartments rather than the cytoplasm. Whether and to what extent there are transient elevations of free zinc levels in the cytoplasm remains unclear.

Dentate Gyrus - metabolism

Transition Elements - metabolism

Fluorescent Dyes - metabolism

Rats

Male

Chelating Agents - pharmacology

Hippocampus - drug effects

Rats, Sprague-Dawley

Mice, Knockout

Hippocampus - metabolism

Animals

Dentate Gyrus - drug effects

Female

Mice

In Vitro Techniques

Polycyclic Compounds - metabolism

Details

Title: Subtitle: Differential transition metal uptake and fluorescent probe localization in hippocampal slices
Creators: Alan R Kay - Dept. Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA. alan-kay@uiowa.edu
Sean M Rumschik
Resource Type: Journal article
Publication Details: Metallomics, Vol.3(8), pp.829-837
Publisher: England
DOI: 10.1039/c1mt00024a
PMID: 21681308
ISSN: 1756-5901
eISSN: 1756-591X
Grant note: NS47508 / NINDS NIH HHS P30 ES0560 / NIEHS NIH HHS
Language: English
Date published: 08/2011
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984068244702771

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