Journal article
Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome
Clinical and experimental nephrology, Vol.20(2), pp.273-283
04/2016
DOI: 10.1007/s10157-015-1162-7
PMID: 26351173
Abstract
We performed a discovery phase of urinary proteomic profile in children with idiopathic nephrotic syndrome and validated selected biomarkers.
Urinary proteomic profile was performed using isobaric tags for relative and absolute quantitation labeling, coupled with liquid chromatography-matrix assisted laser desorption and ionization analysis. Validation of biomarkers apolipoprotein A1, alpha 2 macroglobulin, orosomucoid 2, retinol binding protein 4 and leucine-rich alpha 2-glycoprotein 1 was done by enzyme-linked immunosorbent assay.
Apolipoprotein A1 levels of <0.48 µg/mg of creatinine-differentiated steroid-resistant nephrotic syndrome (SRNS) from first episode nephrotic syndrome, area under curve (AUC) [0.99 (CI 0.9-1.0), 100 % sensitivity and 100 % specificity] and a value of <0.24 µg/mg of creatinine could differentiate SRNS from frequently relapsing nephrotic syndrome/steroid dependent nephrotic syndrome [AUC 0.99 (CI 0.9-1.0), 100 % sensitivity and 100 % specificity]. Alpha 2 macroglobulin could differentiate children with SRNS-focal segmental glomerulosclerosis (FSGS) from SRNS-minimal change disease (MCD) at values >3.3 µg/mg of creatinine [AUC 0.84 (CI 0.62-1.0), 90 % sensitivity and 85 % specificity]. Orosomucoid 2 >1.81 µg/mg of creatinine could distinguish SRNS-FSGS from SRNS-MCD [AUC 0.84 (CI 0.62-1.0), sensitivity 90 % and specificity 85.5 %]. RBP 4 value of >1.54 µg/mg of creatinine differentiated SRNS-FSGS from SRNS-MCD [AUC 0.87 (CI 0.68-1.0), sensitivity 90 % and specificity 85.7 %].
Lower level of apolipoprotein A1 in urine is suggestive of SRNS. Alpha 2 macroglobulin, retinol binding protein 4 and orosomucoid 2 are markers associated with FSGS, with alpha 2 macroglobulin being most predictive.
Details
- Title: Subtitle
- Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome
- Creators
- C P Suresh - Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia HospitalAbhijeet Saha - Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia HospitalManpreet Kaur - Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia HospitalRitesh Kumar - Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia HospitalN K Dubey - Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia HospitalTrayambak Basak - Institute of Genomics and Integrative BiologyVinay Singh Tanwar - Institute of Genomics and Integrative BiologyGaurav Bhardwaj - Institute of Genomics and Integrative BiologyShantanu Sengupta - Academy of Scientific and Innovative ResearchVineeta Vijay Batra - Govind Ballabh Pant HospitalAshish Datt Upadhyay - All India Institute of Medical Sciences
- Resource Type
- Journal article
- Publication Details
- Clinical and experimental nephrology, Vol.20(2), pp.273-283
- DOI
- 10.1007/s10157-015-1162-7
- PMID
- 26351173
- ISSN
- 1342-1751
- eISSN
- 1437-7799
- Language
- English
- Date published
- 04/2016
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984359863002771
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