Journal article
Differentiating Branch Duct and Mixed IPMN in Endoscopically Collected Pancreatic Cyst Fluid via Cytokine Analysis
Gastroenterology research and practice, Vol.2012, 247309
12/25/2012
DOI: 10.1155/2012/247309
PMCID: PMC3543798
PMID: 23326260
Abstract
Background. Differentiating branch duct from mixed intraductal papillary mucinous neoplasm (BD-IPMN) is problematic, but clinically important as mixed IPMNs are managed surgically, while some BD-IPMN may be followed. Inflammatory mediator proteins (IMPs) have been implicated in acute and chronic inflammatory and malignant pancreatic diseases. Aim. To compare IMP profile of pancreatic cyst fluid collected endoscopically from BD-IPMN and mixed IPMN. Methods. Pancreatic cyst fluid from ten patients (5 BD-IPMN and 5 mixed IPMN) was collected by endoscopic ultrasound-guided fine needle aspiration or endoscopic retrograde cholangiopancreatography. Concentrations of 89 IMPs in these samples were determined using a multiplexed bead-based microarray protein assay and compared between BD-IPMN and mixed IPMN. Results. Eighty-six of 89 IMPs were detected in at least one of the 10 samples. Fourteen IMPs were detected only in mixed IPMN, while none were only in BD-IPMN. Of these, TGF-β1 was most prevalent, present in 3 of 5 mixed IPMNs. Seventy-two IMPs were detected in both BD-IPMN and mixed IPMNs. Of these, only G-CSF (P<0.05) was present in higher concentrations in mixed IPMNs. Conclusion. TGF-β1 and G-CSF detected in endoscopically collected pancreatic cyst fluid are potential diagnostic biomarkers capable of distinguishing mixed IPMN from BD-IPMN.
Details
- Title: Subtitle
- Differentiating Branch Duct and Mixed IPMN in Endoscopically Collected Pancreatic Cyst Fluid via Cytokine Analysis
- Creators
- Linda S Lee - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.eduAndrew M Bellizzi - Department of Pathology University of Iowa Hospitals and Clinics Iowa City IA 52242 USA uiowa.eduPeter A Banks - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.eduNisha I Sainani - Department of Radiology Brigham and Women’s Hospital Boston MA 02115 USA harvard.eduVivek Kadiyala - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.eduShadeah Suleiman - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.eduDarwin L Conwell - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.eduJoao A Paulo - Center for Pancreatic Disease and Division of Gastroenterology Hepatology and Endoscopy Brigham and Women’s Hospital and Department of Medicine Harvard Medical School 75 Francis Street Boston MA 02115 USA harvard.edu
- Contributors
- Massimo Raimondo (Editor)
- Resource Type
- Journal article
- Publication Details
- Gastroenterology research and practice, Vol.2012, 247309
- DOI
- 10.1155/2012/247309
- PMID
- 23326260
- PMCID
- PMC3543798
- NLM abbreviation
- Gastroenterol Res Pract
- ISSN
- 1687-6121
- eISSN
- 1687-630X
- Publisher
- Hindawi Publishing Corporation
- Grant note
- DOI: 10.13039/100007827, name: American College of Gastroenterology, award: F32 DK085835-01A1, 5 P30 DK034854-24, 1R21 DK081703-01A2
- Language
- English
- Date published
- 12/25/2012
- Academic Unit
- Pathology
- Record Identifier
- 9984047646402771
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