Differentiation induces up-regulation of plasma membrane Ca2+-ATPase and concomitant increase in Ca2+ efflux in human neuroblastoma cell line IMR-32

Yuriy M USACHEV; Sonja L TOUTENHOOFD; Geoffrey M GOELLNER; Emanuel E STREHLER; Stanley A THAYER

doi:10.1046/j.1471-4159.2001.00169.x

Back

Differentiation induces up-regulation of plasma membrane Ca2+-ATPase and concomitant increase in Ca2+ efflux in human neuroblastoma cell line IMR-32

Journal article

Open access

Peer reviewed

Differentiation induces up-regulation of plasma membrane Ca2+-ATPase and concomitant increase in Ca2+ efflux in human neuroblastoma cell line IMR-32

Yuriy M USACHEV, Sonja L TOUTENHOOFD, Geoffrey M GOELLNER, Emanuel E STREHLER and Stanley A THAYER

Journal of neurochemistry, Vol.76(6), pp.1756-1765

2001

DOI: 10.1046/j.1471-4159.2001.00169.x

PMID: 11259493

Files and links (1)

url

https://doi.org/10.1046/j.1471-4159.2001.00169.xView

Published (Version of record) Open Access

Abstract

Precise regulation of intracellular Ca(2+) concentration ([Ca(2+)](i)) is achieved by the coordinated function of Ca(2+) channels and Ca(2+) buffers. Neuronal differentiation induces up-regulation of Ca(2+) channels. However, little is known about the effects of differentiation on the expression of the plasma membrane Ca(2+)-ATPase (PMCA), the principal Ca(2+) extrusion mechanism in neurons. In this study, we examined the regulation of PMCA expression during differentiation of the human neuroblastoma cell line IMR-32. [Ca(2+)](i) was monitored in single cells using indo-1 microfluorimetry. When the Ca(2+)-ATPase of the endoplasmic reticulum was blocked by cyclopiazonic acid, [Ca(2+)](i) recovery after small depolarization-induced Ca(2+) loads was governed primarily by PMCAs. [Ca(2+)](i) returned to baseline by a process described by a monoexponential function in undifferentiated cells (tau = 52 +/- 4 s; n = 25). After differentiation for 12-16 days, the [Ca(2+)](i) recovery rate increased by more than threefold (tau = 17 +/- 1 s; n = 31). Western blots showed a pronounced increase in expression of three major PMCA isoforms in IMR-32 cells during differentiation, including PMCA2, PMCA3 and PMCA4. These results demonstrate up-regulation of PMCAs on the functional and protein level during neuronal differentiation in vitro. Parallel amplification of Ca(2+) influx and efflux pathways may enable differentiated neurons to precisely localize Ca(2+) signals in time and space.

Cell Physiology

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Molecular and cellular biology

Cell differentiation, maturation, development, hematopoiesis

Details

Title: Subtitle: Differentiation induces up-regulation of plasma membrane Ca2+-ATPase and concomitant increase in Ca2+ efflux in human neuroblastoma cell line IMR-32
Creators: Yuriy M USACHEV - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, United States
Sonja L TOUTENHOOFD - Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, United States
Geoffrey M GOELLNER - Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, United States
Emanuel E STREHLER - Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, United States
Stanley A THAYER - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, United States
Resource Type: Journal article
Publication Details: Journal of neurochemistry, Vol.76(6), pp.1756-1765
DOI: 10.1046/j.1471-4159.2001.00169.x
PMID: 11259493
NLM abbreviation: J Neurochem
ISSN: 0022-3042
eISSN: 1471-4159
Publisher: Blackwell; Oxford
Language: English
Date published: 2001
Academic Unit: Iowa Neuroscience Institute; Anesthesia; Neuroscience and Pharmacology
Record Identifier: 9984040554502771

Metrics

21 Record Views

39 Times Cited - Web of Science