Differentiation of central memory CD8 T cells is independent of CD62L-mediated trafficking to lymph nodes

Thomas C Wirth; Vladimir P Badovinac; Lichao Zhao; Morris O Dailey; John T Harty

doi:10.4049/jimmunol.0803315

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Differentiation of central memory CD8 T cells is independent of CD62L-mediated trafficking to lymph nodes

Journal article

Peer reviewed

Differentiation of central memory CD8 T cells is independent of CD62L-mediated trafficking to lymph nodes

Thomas C Wirth, Vladimir P Badovinac, Lichao Zhao, Morris O Dailey and John T Harty

The Journal of immunology (1950), Vol.182(10), pp.6195-6206

05/15/2009

DOI: 10.4049/jimmunol.0803315

PMID: 19414773

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Abstract

CD62L (L-selectin) is a key regulator of T cell trafficking, and its surface expression on activated T cells is modulated to control T cell access to lymph nodes after acute infections. In memory T cells, CD62L is the most frequently used marker to define central memory T cells, a population that provides enhanced protection against most, but not all, pathogens. Early access of CD62L(pos) effector T cells to lymph nodes has been proposed to result in preferential central memory T cell differentiation, but direct proof for the involvement of lymph node homing in memory T cell differentiation is lacking. In this study, we show that central memory lineage commitment in CD8 T cells is unaltered by enhanced entry into lymph nodes as a result of constitutive CD62L expression, and that equal numbers of effector and central memory CD8 T cells develop in the absence of CD62L-mediated lymph node trafficking. Our results suggest that CD62L is not a deterministic marker of central memory T cell differentiation, thus providing new insight into the process of memory CD8 T cell generation.

CD8-Positive T-Lymphocytes - cytology

Mice, Inbred C57BL

Mice, Transgenic

Adoptive Transfer

Lymph Nodes - immunology

Lymph Nodes - cytology

L-Selectin - metabolism

Cell Differentiation - immunology

L-Selectin - immunology

Lymphocyte Activation - immunology

Animals

Immunologic Memory

Mice

CD8-Positive T-Lymphocytes - immunology

Chemotaxis, Leukocyte - immunology

Details

Title: Subtitle: Differentiation of central memory CD8 T cells is independent of CD62L-mediated trafficking to lymph nodes
Creators: Thomas C Wirth - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Vladimir P Badovinac
Lichao Zhao
Morris O Dailey
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.182(10), pp.6195-6206
DOI: 10.4049/jimmunol.0803315
PMID: 19414773
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Language: English
Date published: 05/15/2009
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047743902771

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