Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics

Meenakshi Kushwaha; Xueliang Pan; Julie A Holloway; Isabelle L Denry

doi:10.1016/j.dental.2011.10.010

Back

Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics

Journal article

Peer reviewed

Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics

Meenakshi Kushwaha, Xueliang Pan, Julie A Holloway and Isabelle L Denry

Dental materials, Vol.28(3), pp.252-260

03/2012

DOI: 10.1016/j.dental.2011.10.010

PMCID: PMC3279629

PMID: 22078764

View Online

Abstract

Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a niobium-doped fluorapatite-based glass-ceramic (FAp). The glass was prepared by twice melting at 1525°C for 3h, and cast into cylindrical ingots later sectioned into discs and heat-treated to promote crystallization of fluorapatite submicrometer crystals. Tissue culture polystyrene (TCP) was used as control. The surface of the FAp discs was either left as-heat treated, ground or etched. Initial cell attachment was assessed at 3h. Proliferation and alkaline phosphatase (ALP) expression data were collected at days 1, 4, and 8. Cell morphology was examined using SEM, at days 2 and 4. Mineralization was evaluated by Alizarin Red staining and SEM. Initial cell attachment on as heat-treated, etched, or ground surfaces was similar to that of the positive control group (p>0.05). The percentage of area covered by living cells increased significantly on as heat-treated, etched, or ground surfaces between days 1 and 8 (p<0.05). There was no significant difference among groups in cell coverage at day 8, compared to TCP control. SEM revealed well spread polygonal cells with numerous filopodia, either attached to the ceramic surface or connected to neighboring cells. ALP expression at day 8 was significantly higher in osteogenic media compared to growth media on both FAp and control. FAp discs stained positively with Alizarin Red and calcium-rich mineralized granules associated with fibrils were observed by SEM at day 35. hMSCs displayed excellent attachment, proliferation, and differentiation on niobium-doped FAp glass-ceramic.

Glass-ceramic

Mineralization

Biocompatibility

Surface topography

Proliferation

Differentiation

Adhesion

Niobium

Fluorapatite

Mesenchymal stem cells

Details

Title: Subtitle: Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics
Creators: Meenakshi Kushwaha - The University of Iowa, College of Dentistry, Dows Institute for Dental Research, 801 Newton Road, Iowa City, IA 52242-1010, USA
Xueliang Pan - Center for Biostatistics, The Ohio State University, Columbus, OH 43210, USA
Julie A Holloway - The University of Iowa, College of Dentistry, Dows Institute for Dental Research, 801 Newton Road, Iowa City, IA 52242-1010, USA
Isabelle L Denry - The University of Iowa, College of Dentistry, Dows Institute for Dental Research, 801 Newton Road, Iowa City, IA 52242-1010, USA
Resource Type: Journal article
Publication Details: Dental materials, Vol.28(3), pp.252-260
DOI: 10.1016/j.dental.2011.10.010
PMID: 22078764
PMCID: PMC3279629
NLM abbreviation: Dent Mater
ISSN: 0109-5641
eISSN: 1879-0097
Publisher: Elsevier Ltd
Language: English
Date published: 03/2012
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Dental Research
Record Identifier: 9984065807102771

Metrics

13 Record Views

53 Times Cited - Web of Science