Journal article
Differentiation of mumps virus strains by polymerase chain reaction and silver stained single-strand conformation polymorphism analysis
Zhonghua shi yan he lin chuang bing du xue za ji, Vol.11(4), pp.315-318
12/1997
PMID: 15617236
Abstract
The cDNA fragments amplified through nested RT-PCR from the small hydrophobic (SH) protein gene and its flanking region of the five wild mumps viruses and Enders strain were subjected to single-strand conformation polymorphism (SSCP) analysis by using silver staining. These five wild isolates could be divided into three SSCP patterns and they were differentiated from the Enders strain. Two wild strains which have 3.4% heterology of the nucleotide acid sequence could be distinguished by the method. The results were well correlated with sequence analysis of the SH gene segments, indicating high applicability of the silver staining SSCP analysis for differentiation of mumps virus strains. Furthermore, the method, which is not only quick, sensitive, safe and reliable, but also reproducible and inexpensive, can be used in the molecular epidemiology study of mumps viruses.
Details
- Title: Subtitle
- Differentiation of mumps virus strains by polymerase chain reaction and silver stained single-strand conformation polymorphism analysis
- Creators
- L Wu - Lanzhou Institute of Biological Products, Lanzhou 730046X NingY LiZ Bai
- Resource Type
- Journal article
- Publication Details
- Zhonghua shi yan he lin chuang bing du xue za ji, Vol.11(4), pp.315-318
- Publisher
- China
- PMID
- 15617236
- ISSN
- 1003-9279
- Language
- Chinese
- Date published
- 12/1997
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001227802771
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