Differentiation of preosteoblasts is affected by implant surface microtopographies

Galen B Schneider; Rebecca Zaharias; Denise Seabold; John Keller; Clark Stanford

doi:10.1002/jbm.a.30016

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Differentiation of preosteoblasts is affected by implant surface microtopographies

Journal article

Peer reviewed

Differentiation of preosteoblasts is affected by implant surface microtopographies

Galen B Schneider, Rebecca Zaharias, Denise Seabold, John Keller and Clark Stanford

Journal of biomedical materials research. Part A, Vol.69(3), pp.462-468

06/01/2004

DOI: 10.1002/jbm.a.30016

PMID: 15127393

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Abstract

Osteogenesis involves the recruitment of multipotent mesenchymal cells and the progressive differentiation of these cells into osteoblasts. The transcription factor Runx2 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if preosteoblast cell differentiation associated with Runx2 and osteocalcin gene expression was influenced by implant surface microtopography. Human embryonic palatal mesenchymal cells (HEPM cells) were cultured on grooved or roughened cpTi implant discs. Cell phenotypes were evaluated with epifluorescent microscopy. Real-time PCR was used for quantitative analysis of Runx2 and osteocalcin gene expression. HEPM cells mineralized when grown on rough and grooved implant surfaces relative to tissue culture plastic. Real-time PCR showed significant (p < 0.05) increases in Runx2 and osteocalcin gene expression in cells cultured on rough and grooved implant microtopographies. These results suggest that preosteoblast cell differentiation is affected by implant surface microtopographies during osseointegration of dental implants.

Anthraquinones - metabolism

Biocompatible Materials - metabolism

Biocompatible Materials - chemistry

Humans

Osteocalcin - genetics

Titanium - chemistry

Molecular Sequence Data

Transcription Factor AP-2

Cell Culture Techniques - methods

DNA-Binding Proteins - metabolism

Mesenchymal Stromal Cells - cytology

Base Sequence

Surface Properties

Core Binding Factor Alpha 1 Subunit

Osteoblasts - cytology

Cell Differentiation - physiology

Mesenchymal Stromal Cells - physiology

Osteocalcin - metabolism

Osteoblasts - physiology

Cells, Cultured

Gene Expression Regulation

Prostheses and Implants

Materials Testing

Transcription Factors - genetics

DNA-Binding Proteins - genetics

Transcription Factors - metabolism

Coloring Agents - metabolism

Details

Title: Subtitle: Differentiation of preosteoblasts is affected by implant surface microtopographies
Creators: Galen B Schneider - Dows Institute for Dental Research and the Department of Prosthodontics, N402, University of Iowa College of Dentistry, Iowa City, Iowa 52242
Rebecca Zaharias
Denise Seabold
John Keller
Clark Stanford
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part A, Vol.69(3), pp.462-468
DOI: 10.1002/jbm.a.30016
PMID: 15127393
NLM abbreviation: J Biomed Mater Res A
ISSN: 1549-3296
eISSN: 1552-4965
Publisher: United States
Grant note: R03DE014269 / NIDCR NIH HHS P60DE13076 / NIDCR NIH HHS
Language: English
Date published: 06/01/2004
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Graduate College Admin and Gen; Anatomy and Cell Biology; President; Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984065720902771

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