Journal article
Differentiation of small bowel and pancreatic neuroendocrine tumors by gene-expression profiling
Surgery, Vol.152(6), pp.998-1007
12/2012
DOI: 10.1016/j.surg.2012.08.040
PMCID: PMC3502942
PMID: 23158174
Abstract
Between 10% and 20% of patients with neuroendocrine tumors (NETs) present with metastases of unknown primary site. Because knowledge of the primary site has important implications for treatment, we set out to define gene-expression profiles to differentiate between small-bowel NETs (SBNETs) and pancreatic NETs (PNETs).
RNA was extracted from tumor and normal tissues in 11 patients with SBNETs and 15 patients with PNETs, and qPCR was performed for 367 GPCR genes. Differentially expressed genes were identified using the RT2 Profiler. Whole genome expression analysis was performed on 11 SBNETs, 5 PNETS, and corresponding normal tissues. Statistical significance was evaluated by the Student t test and ANOVA.
Whole-genome analysis revealed 173 significantly differentially expressed genes in SBNETs and normal tissues and in 52 in PNETs. GPCR arrays identified 28 genes in SBNETs and 18 in PNETs, with significant expression differences from normal tissues. In all SBNETs, 2 genes were significantly upregulated by more than fivefold: OXTR and GPR113. No PNETs shared this profile, whereas 73% had a greater than fivefold downregulation of ADORA1 and SCTR. These genes also allowed for determination of the primary site in 8 of 10 liver metastases.
Differential expression patterns using as few as 2 to 4 GPCR genes successfully discriminated primary sites in small bowel and pancreatic NETs.
Details
- Title: Subtitle
- Differentiation of small bowel and pancreatic neuroendocrine tumors by gene-expression profiling
- Creators
- Jennifer C Carr - Department of Surgery, University of Iowa Carver College of Medicine, Iowa City, IAErin A Boese - University of Iowa, Ophthalmology and Visual SciencesPhilip M Spanheimer - Department of Surgery, University of Iowa Carver College of Medicine, Iowa City, IAFadi S Dahdaleh - Department of Surgery, University of Iowa Carver College of Medicine, Iowa City, IAMolly Martin - University of Iowa, Pharmacy Practice and ScienceDaniel Calva - Department of Surgery, University of Iowa Carver College of Medicine, Iowa City, IABlanca Schafer - Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IADavid M Thole - University of Iowa, Business AnalyticsTerry Braun - University of Iowa, Roy J. Carver Department of Biomedical EngineeringThomas M O'Dorisio - University of Iowa, Internal MedicineM. Sue O'Dorisio - University of Iowa, Stead Family Department of PediatricsJames R Howe - University of Iowa, Surgery
- Resource Type
- Journal article
- Publication Details
- Surgery, Vol.152(6), pp.998-1007
- DOI
- 10.1016/j.surg.2012.08.040
- PMID
- 23158174
- PMCID
- PMC3502942
- NLM abbreviation
- Surgery
- ISSN
- 0039-6060
- eISSN
- 1532-7361
- Publisher
- Mosby, Inc
- Grant note
- University of Iowa Institute for Clinical and Translational Science CA148062-01 / NIH T32
- Language
- English
- Date published
- 12/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Surgery; Pharmacy Practice and Science; Endocrinology and Metabolism; Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9984107735902771
Metrics
24 Record Views