Journal article
Differing effects of inducers of differentiation on the ribonucleotide pool sizes of friend leukemia cells
Life sciences (1973), Vol.17(8), pp.1287-1290
1975
DOI: 10.1016/0024-3205(75)90139-3
PMID: 1238891
Abstract
Friend leukemia cells differentiate when any one of a variety of cryoprotective agents are added to their suspension culture media. While dimethyl sulfoxide (DMSO), dimethylacetamide (DMA) and tetramethylurea (TMU) induce a comparable degree of erythroid maturation their effects on the acid soluble ribonucleotide pools differ. DMSO induced differentiation is accompanied by a 70–80% reduction in pool size while DMA and TMA cause only a 15–20% reduction. Furthermore, inhibition of DMSO-induced differentiation by BUdR does not prevent the DMSO-induced reduction in pool size. These data suggest that the decline in pool size is a toxic effect unrelated to differentiation and these effects should be considered when the biological effects of DMSO-induced differentiation are considered.
Details
- Title: Subtitle
- Differing effects of inducers of differentiation on the ribonucleotide pool sizes of friend leukemia cells
- Creators
- Harvey D. Preisler - Roswell Park Cancer InstituteYoucef M. Rustum - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.17(8), pp.1287-1290
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0024-3205(75)90139-3
- PMID
- 1238891
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Language
- English
- Date published
- 1975
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359853102771
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