Diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon, NeuroVue™ Red and NeuroVue™ Green and their use for double and triple labeling of neuronal profile

B Fritzsch; K.A Muirhead; Feng Feng; B.D Gray; B.M Ohlsson-Wilhelm

doi:10.1016/j.brainresbull.2005.05.016

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Diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon, NeuroVue™ Red and NeuroVue™ Green and their use for double and triple labeling of neuronal profile

B Fritzsch, K.A Muirhead, Feng Feng, B.D Gray and B.M Ohlsson-Wilhelm

Brain research bulletin, Vol.66(3), pp.249-258

2005

DOI: 10.1016/j.brainresbull.2005.05.016

PMCID: PMC1513641

PMID: 16023922

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Abstract

We describe here diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon (near infrared), NeuroVue™ Red and NeuroVue™ Green. Using pair-wise comparisons between the new dyes and existing dyes (DiI, DiA, DiD, DiO, PKH2, PKH26) applied to the left and the right side of fixed spinal cord preparations, we show that NeuroVue™ Maroon (excitation maximum 647\nnm) surpasses all other dyes in this study in signal to noise ratio. We also present data showing the utility of these new dyes for both double labeling and triple labeling in combination with each other or existing lipophilic tracers. Using mice bearing the PLP–eGFP transgene, we demonstrate that either NeuroVue™ Maroon or NeuroVue™ Red can readily be combined with eGFP labeling. Double labeling experiments using NeuroVue™ Red and eGFP allowed us to demonstrate that every fiber in the neonatal ear is surrounded by developing Schwann cells.

Confocal Microscopy

Lipophilic tracers

Diffusion rates

Imaging

Details

Title: Subtitle: Diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon, NeuroVue™ Red and NeuroVue™ Green and their use for double and triple labeling of neuronal profile
Creators: B Fritzsch - Creighton University, Department of Biomedical Sciences, Omaha, NE 68178, USA
K.A Muirhead - SciGro Inc., Malvern, PA, USA
Feng Feng - Creighton University, Department of Biomedical Sciences, Omaha, NE 68178, USA
B.D Gray - PTI Research, Exton, PA, USA
B.M Ohlsson-Wilhelm - SciGro Inc., Malvern, PA, USA
Resource Type: Journal article
Publication Details: Brain research bulletin, Vol.66(3), pp.249-258
DOI: 10.1016/j.brainresbull.2005.05.016
PMID: 16023922
PMCID: PMC1513641
NLM abbreviation: Brain Res Bull
ISSN: 0361-9230
eISSN: 1873-2747
Publisher: Elsevier Inc
Language: English
Date published: 2005
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070970402771

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