Journal article
Dimeric Proanthocyanidins on the Stability of Dentin and Adhesive Biointerfaces
Journal of dental research, Vol.99(2), pp.175-181
02/2020
DOI: 10.1177/0022034519892959
PMCID: PMC7431887
PMID: 31826696
Abstract
A dentin biomodification strategy with selective proanthocyanidin (PAC)-enriched extracts reinforces dentin and dentin-resin interfaces. Enrichment of the extracts according to the degree of polymerization allows exploration of bioactive principles of PACs and structure-activity relationships. This study investigated the sustained dentin matrix biomodification and dentin-resin bioadhesion of 2 fractions consisting exclusively of B-type PAC dimers with or without a single galloyl motif (specifically, DIMER
and DIMER
) and their precursor material, enriched grape seed extract (e-GSE;
). The biomodification potential was determined by long-term evaluation of the apparent modulus of elasticity and collagen solubility (hydroxyproline release). Chemical characterization of the dentin matrix was performed by attenuated total reflectance-Fourier-transform infrared spectroscopy. The bioadhesive properties were assessed by a microtensile bond strength test at different time points, and macro-hybrid layers were produced to verify the degree of conversion of the adhesive resin. Fractions consisting of DIMER
, DIMER
, and their precursor, e-GSE, increased the modulus of elasticity at all time points and reduced collagen degradation. Specimens treated with DIMER
remained stable throughout 12 mo of storage, whereas a significant drop in the modulus of elasticity was observed for the DIMER
and e-GSE groups at 6 mo. The fractions and precursor did not affect the degree of resin conversion at the hybrid layer. Changes in infrared resonances corresponding to collagen cross-links in the dentin matrix occurred for all treatments. Higher bond strength was observed for dentin treated with e-GSE as compared with DIMER
and DIMER
; all biointerfaces remained stable after 12 mo. Nongalloylated PACs mediate stable dentin biomodification, which includes protective activity against collagen degradation and reinforcement of the anchoring dentin matrix. Collectively, PACs with a higher degree of oligomerization offer a robust bioadhesion between the hydrophilic dentin matrix and the hydrophobic adhesive.
Details
- Title: Subtitle
- Dimeric Proanthocyanidins on the Stability of Dentin and Adhesive Biointerfaces
- Creators
- A A Leme-Kraus - University of Illinois at ChicagoR S Phansalkar - University of Illinois at ChicagoM C Dos Reis - University of Illinois at ChicagoB Aydin - University of Illinois at ChicagoA B S Sousa - University of Illinois at ChicagoY Alania - University of Illinois at ChicagoJ McAlpine - University of Illinois at ChicagoS N Chen - University of Illinois at ChicagoG F Pauli - University of Illinois at ChicagoA K Bedran-Russo - University of Illinois at Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.99(2), pp.175-181
- DOI
- 10.1177/0022034519892959
- PMID
- 31826696
- PMCID
- PMC7431887
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Grant note
- R01 DE021040 / NIDCR NIH HHS R56 DE021040 / NIDCR NIH HHS
- Language
- English
- Date published
- 02/2020
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984367718202771
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