Journal article
Dimethylthiourea, but not dimethylsulfoxide, reduces canine myocardial infarct size
Free radical biology & medicine, Vol.7(1), pp.53-58
1989
DOI: 10.1016/0891-5849(89)90100-7
PMID: 2502484
Abstract
We studied the effect of treatment with two diffusible, low molecular weight scavengers of toxic oxygen metabolites, dimethylthiourea (DMTU) and dimethylsulfoxide (DMSO), on canine infarcts caused by 90 min of ischemia and 3 h of reperfusion. Infarct size was determined by incubating ventricular slices with triphenyl tetrazolium chloride. Areas at risk were determined by autoradiography of
99Tc microspheres injected in vivo during ischemia and were similar (
p > 0.05) in DMSO, and saline treated dogs. However, the ratio of infarct size to area at risk was reduced (
p < 0.05) in dogs treated 30 min before reperfusion with 500 mg/kg DMTU (31.1 ± 4.6%,
n = 9) compared with saline treated dogs (53.4 ± 4.6%
n = 9). In contrast, the ratio of infarct size to area at risk was not significantly different (
p > 0.05) in dogs treated with 2000 mg/kg DMSO 30 min before reperfusion (43.7 ± 4.3%) compared to saline treated dogs. The serum concentration of DMTU (4.5 mM) was one-tenth that of DMSO (48 mM) in early reperfusion. Therefore, DMTU but not DMSO protected against post-ischemic cardiac reperfusion injury.
Details
- Title: Subtitle
- Dimethylthiourea, but not dimethylsulfoxide, reduces canine myocardial infarct size
- Creators
- Stephen J. Portz - University of Colorado DenverEdward J. Lesnefsky - University of Colorado DenverKaryl M. VanBenthuysen - University of Colorado DenverJohn E. Repine - University of Colorado DenverNatalie B. Parker - University of Colorado DenverIvan F. McMurtry - University of Colorado DenverLawrence D. Horwitz - University of Colorado Denver
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.7(1), pp.53-58
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0891-5849(89)90100-7
- PMID
- 2502484
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Language
- English
- Date published
- 1989
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656605502771
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