Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis

Asgar Zaheer; Shailendra K Sahu; Yanghong Wu; Ashna Zaheer; Joel Haas; Kiwhoon Lee; Baoli Yang

doi:10.1016/j.brainres.2007.01.075

Back

Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis

Journal article

Peer reviewed

Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis

Asgar Zaheer, Shailendra K Sahu, Yanghong Wu, Ashna Zaheer, Joel Haas, Kiwhoon Lee and Baoli Yang

Brain research, Vol.1144(1), pp.239-247

2007

DOI: 10.1016/j.brainres.2007.01.075

PMCID: PMC1899479

PMID: 17316572

Files and links (1)

url

http://doi.org/10.1016/j.brainres.2007.01.075View

Open Access

Abstract

Pro-inflammatory cytokines/chemokines are implemented in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model with clinical and pathological similarities to multiple sclerosis. We have previously shown that over-expression of glia maturation factor (GMF) in glial cells cause excessive production and secretion of pro-inflammatory cytokines/chemokines sufficient to destroy the myelin-forming oligodendroglial cell in vitro. In this present investigation, we evaluate the expression of pro-inflammatory mediators in the central nervous system (CNS) of GMF+/+ (wild type) mice and GMF−/− (GMF-knockout) mice at the peak of EAE induced by immunization with MOG 35–55 peptide. GMF+/+ (Wt) mice developed severe EAE with a maximal mean clinical score of 3.6 ± 0.5 by day 16 post-immunization, whereas GMF-KO mice showed significantly delayed EAE with an average onset on day 26 pi with reduced mean clinical score of 1.3 ± 0.3. Three of fifteen Wt mice as compared to none of GMF-KO mice died of EAE. Encephalitogenic cells from Wt mice transferred to recipient GMF-KO mice caused very mild and with low incidence of EAE. We determined the differences in the expression of cytokines, IFN-γ, TNF-α, IL-1 β, IL-6, IL-4, IL-10, and chemokines, MIP-1, MIP-2, IP-10, MCP-1, GM-CSF mRNA by quantitative real-time RT–PCR in brain and spinal cord. Our results demonstrate significantly low levels of pro-inflammatory cytokines/chemokines in the CNS of GMF-KO mice and increased expression in Wt mice with EAE. Our data suggest that GMF play a critical role in CNS inflammation.

Inflammation

Cytokine/Chemokine

Experimental autoimmune encephalomyelitis (EAE)

Glia maturation factor (GMF)

Multiple sclerosis (MS)

Myelin oligodendrocyte glycoprotein (MOG)

Details

Title: Subtitle: Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis
Creators: Asgar Zaheer - University of Iowa, Neurology
Shailendra K Sahu - Division of Neurochemistry and Neurobiology, Department of Neurology, The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
Yanghong Wu - Division of Neurochemistry and Neurobiology, Department of Neurology, The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
Ashna Zaheer - Department of Obstetric and Gynecology, University of Iowa, Iowa City, IA 52242, USA
Joel Haas - Veterans Affair Medical Center, Iowa City, IA 52242, USA
Kiwhoon Lee - Division of Neurochemistry and Neurobiology, Department of Neurology, The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
Baoli Yang - University of Iowa, BioVentures Center
Resource Type: Journal article
Publication Details: Brain research, Vol.1144(1), pp.239-247
DOI: 10.1016/j.brainres.2007.01.075
PMID: 17316572
PMCID: PMC1899479
NLM abbreviation: Brain Res
ISSN: 0006-8993
eISSN: 1872-6240
Publisher: Elsevier B.V
Language: English
Date published: 2007
Academic Unit: Neurology; BioVentures Center; Obstetrics and Gynecology
Record Identifier: 9983557773602771

Metrics

19 Record Views

37 Times Cited - Web of Science