Journal article
Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-gamma in myelin from glia maturation factor-deficient mice
Neuroscience research, Vol.58(2), pp.156-163
06/01/2007
DOI: 10.1016/j.neures.2007.02.010
PMCID: PMC1992520
PMID: 17383764
Abstract
In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin isolated from control wild type (WT) and glia maturation factor (GMF)-deficient (GMF-KO) mice. GMF was not detected in isolated myelin from WT and GMF-KO mice although it is present in brains of WT mice. Our results show that calcium-dependent neutral protease activity caused significantly elevated degradation of 18.5 and/or 17.5 kDa isoforms of MBP in WT myelin treated with RmAb or IFN-γ. In contrast, MBP degradation in isolated myelin from GMF-KO mice remained unaffected following treatment with RmAb, IFN-γ, or GM-CSF. Neither the 14 kDa isoform of MBP nor proteolipid protein (PLP) showed an elevated degradation compared to controls. A virtual absence of GM-CSF, TNF-α and IFN-γ in GMF-KO brain compared to WT was also evident when the animals were challenged with MOG 35-55. Additionally, the myelin from GMF-KO mice showed difference in distribution of myelin oligodendrocyte glycoprotein (MOG) and β-tubulin in a sucrose density gradient myelin–axolemmal fractions compared to WT. Taken together, our data suggests a role for GMF in the biochemical organization of myelin and thereby its effect on MBP degradation induced by RmAb and IFN-γ.
Details
- Title: Subtitle
- Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-gamma in myelin from glia maturation factor-deficient mice
- Creators
- Krishnakumar MenonYanghong WuJoel HaasShailendra K. SahuBaoli Yang - University of IowaAsgar Zaheer
- Resource Type
- Journal article
- Publication Details
- Neuroscience research, Vol.58(2), pp.156-163
- DOI
- 10.1016/j.neures.2007.02.010
- PMID
- 17383764
- PMCID
- PMC1992520
- NLM abbreviation
- Neurosci Res
- ISSN
- 0168-0102
- Publisher
- Elsevier BV
- Language
- English
- Date published
- 06/01/2007
- Academic Unit
- Neurology; BioVentures Center; Obstetrics and Gynecology
- Record Identifier
- 9983557416602771
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