Direct Parabrachial–Cortical Connectivity

Fillan Grady; Lila Peltekian; Gabrielle Iverson; Joel C Geerling

doi:10.1093/cercor/bhaa072

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Direct Parabrachial–Cortical Connectivity

Journal article

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Direct Parabrachial–Cortical Connectivity

Fillan Grady, Lila Peltekian, Gabrielle Iverson and Joel C Geerling

Cerebral cortex (New York, N.Y. 1991), Vol.30(9), pp.4811-4833

07/30/2020

DOI: 10.1093/cercor/bhaa072

PMID: 32383444

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Abstract

Abstract The parabrachial nucleus (PB) in the upper brain stem tegmentum includes several neuronal subpopulations with a wide variety of connections and functions. A subpopulation of PB neurons projects axons directly to the cerebral cortex, and limbic areas of the cerebral cortex send a return projection directly to the PB. We used retrograde and Cre-dependent anterograde tracing to identify genetic markers and characterize this PB–cortical interconnectivity in mice. Cortical projections originate from glutamatergic PB neurons that contain Lmx1b (81%), estrogen receptor alpha (26%), and Satb2 (20%), plus mRNA for the neuropeptides cholecystokinin (Cck, 48%) and calcitonin gene-related peptide (Calca, 13%), with minimal contribution from FoxP2+ PB neurons (2%). Axons from the PB produce an extensive terminal field in an unmyelinated region of the insular cortex, extending caudally into the entorhinal cortex, and arcing rostrally through the dorsolateral prefrontal cortex, with a secondary terminal field in the medial prefrontal cortex. In return, layer 5 neurons in the insular cortex and other prefrontal areas, along with a dense cluster of cells dorsal to the claustrum, send a descending projection to subregions of the PB that contain cortically projecting neurons. This information forms the neuroanatomical basis for testing PB–cortical interconnectivity in arousal and interoception.

medial prefrontal

ascending reticular activating system

frontoinsular

consciousness

coma

dysgranular insular

Details

Title: Subtitle: Direct Parabrachial–Cortical Connectivity
Creators: Fillan Grady - Department of Neurology, Iowa Neuroscience Institute, University of Iowa, Iowa City, IA 52246, USA
Lila Peltekian - Department of Neurology, Iowa Neuroscience Institute, University of Iowa, Iowa City, IA 52246, USA
Gabrielle Iverson - Department of Neurology, Iowa Neuroscience Institute, University of Iowa, Iowa City, IA 52246, USA
Joel C Geerling - Department of Neurology, Iowa Neuroscience Institute, University of Iowa, Iowa City, IA 52246, USA
Resource Type: Journal article
Publication Details: Cerebral cortex (New York, N.Y. 1991), Vol.30(9), pp.4811-4833
DOI: 10.1093/cercor/bhaa072
PMID: 32383444
NLM abbreviation: Cereb Cortex
ISSN: 1047-3211
eISSN: 1460-2199
Publisher: Oxford University Press
Grant note: name: University of Iowa Center for Aging; DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke, award: NS099425
Language: English
Date published: 07/30/2020
Academic Unit: Neurology; Iowa Neuroscience Institute
Record Identifier: 9984070954302771

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