Direct cancer tissue proteomics : a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues

S.-I HWANG; J THUMAR; D. H LUNDGREN; K REZAUL; V MAYYA; L WU; J ENG; M. E WRIGHT; D. K HAN

doi:10.1038/sj.onc.1209755

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Direct cancer tissue proteomics : a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues

Journal article

Peer reviewed

Direct cancer tissue proteomics : a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues

S.-I HWANG, J THUMAR, D. H LUNDGREN, K REZAUL, V MAYYA, L WU, J ENG, M. E WRIGHT and D. K HAN

Oncogene, Vol.26(1), pp.65-76

2007

DOI: 10.1038/sj.onc.1209755

PMID: 16799640

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Abstract

Successful treatment of multiple cancer types requires early detection and identification of reliable biomarkers present in specific cancer tissues. To test the feasibility of identifying proteins from archival cancer tissues, we have developed a methodology, termed direct tissue proteomics (DTP), which can be used to identify proteins directly from formalin-fixed paraffin-embedded prostate cancer tissue samples. Using minute prostate biopsy sections, we demonstrate the identification of 428 prostate-expressed proteins using the shotgun method. Because the DTP method is not quantitative, we employed the absolute quantification method and demonstrate picogram level quantification of prostate-specific antigen. In depth bioinformatics analysis of these expressed proteins affords the categorization of metabolic pathways that may be important for distinct stages of prostate carcinogenesis. Furthermore, we validate Wnt-3 as an upregulated protein in cancerous prostate cells by immunohistochemistry. We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types.

Cell Physiology

Tumors

Fundamental and applied biological sciences. Psychology

Tumors of the urinary system

Urinary tract. Prostate gland

Gynecology. Andrology. Obstetrics

Nephrology. Urinary tract diseases

Biological and medical sciences

Molecular and cellular biology

Medical sciences

Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes

Male genital diseases

Details

Title: Subtitle: Direct cancer tissue proteomics : a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues
Creators: S.-I HWANG - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
J THUMAR - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
D. H LUNDGREN - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
K REZAUL - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
V MAYYA - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
L WU - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
J ENG - Fred Hutchinson Cancer Research Center, Seattle, WA, United States
M. E WRIGHT - University of California Davis Genome Center, Davis, CA, United States
D. K HAN - Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States
Resource Type: Journal article
Publication Details: Oncogene, Vol.26(1), pp.65-76
Publisher: Nature Publishing; Basingstoke
DOI: 10.1038/sj.onc.1209755
PMID: 16799640
ISSN: 0950-9232
eISSN: 1476-5594
Language: English
Date published: 2007
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9984025411302771

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