Journal article
Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1–deleted adenoviruses
Nature genetics, Vol.4(1), pp.27-34
05/1993
DOI: 10.1038/ng0593-27
PMID: 7685651
Abstract
We describe the use of a human bronchial xenograft model for studying the efficiency and biology of in vivo gene transfer into human bronchial epithelia with recombinant E1 deleted adenoviruses. All cell types in the surface epithelium except basal cells efficiently expressed the adenoviral transduced recombinant genes, lacZ and CFTR, for 3–5 weeks. Stable transgene expression was associated with high level expression of the early adenoviral gene, E2a, in a subset of transgene expressing cells and virtually undetectable expression of the late adenoviral genes encoding the structural proteins, hexon and fiber. These studies begin to address important issues that relate to safety and in vivo efficacy of recombinant adenoviruses for gene delivery into the human airway.
Details
- Title: Subtitle
- Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1–deleted adenoviruses
- Creators
- John F. Engelhardt - University of Michigan–Ann ArborYiping Yang - University of Michigan–Ann ArborLeslie D. Stratford-Perricaudet - Institut Gustave RoussyEdward D. Allen - University of Michigan–Ann ArborKaren Kozarsky - University of Michigan–Ann ArborMichel Perricaudet - Institut Gustave RoussyJames R. Yankaskas - University of North Carolina at Chapel HillJames M. Wilson - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Nature genetics, Vol.4(1), pp.27-34
- DOI
- 10.1038/ng0593-27
- PMID
- 7685651
- ISSN
- 1061-4036
- eISSN
- 1546-1718
- Language
- English
- Date published
- 05/1993
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984284329602771
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