Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma

Jordan R Witmer; Bailey J Wetherell; Brett A Wagner; Juan Du; Joseph J Cullen; Garry R Buettner

doi:10.1016/j.redox.2016.02.004

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Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma

Journal article

Open access

Peer reviewed

Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma

Jordan R Witmer, Bailey J Wetherell, Brett A Wagner, Juan Du, Joseph J Cullen and Garry R Buettner

Redox biology, Vol.8(C), pp.298-304

08/2016

DOI: 10.1016/j.redox.2016.02.004

PMCID: PMC4776628

PMID: 26928133

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Abstract

Supra-physiological concentrations of ascorbate, vitamin C, in blood, greater than 1mM, achieved through intravenous administration (IV), are being tested in clinical trials to treat human disease, e.g. cancer. These trials need information on the high levels of ascorbate achieved in blood upon IV administration of pharmacological ascorbate so appropriate clinical decisions can be made. Here we demonstrate that in the complex matrix of human blood plasma supra-physiological levels of ascorbate can be quantified by direct UV spectroscopy with use of a microvolume UV-vis spectrophotometer. Direct quantitation of ascorbate in plasma in the range of 2.9mM, lower limit of detection, up to at least 35mM can be achieved without any sample processing, other than centrifugation. This approach is rapid, economical, and can be used to quantify supraphysiological blood levels of ascorbate associated with the use of IV administration of pharmacological ascorbate to treat disease.

Administration, Intravenous

Neoplasms - blood

Humans

Ascorbic Acid - administration & dosage

Ascorbic Acid - pharmacokinetics

Ascorbic Acid - blood

Spectrophotometry, Ultraviolet

Neoplasms - drug therapy

Details

Title: Subtitle: Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma
Creators: Jordan R Witmer - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: jordan.witmer@gmail.com
Bailey J Wetherell - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: wethereb@uni.edu
Brett A Wagner - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: brett-wagner@uiowa.edu
Juan Du - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: juan-du@uiowa.edu
Joseph J Cullen - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: joseph-cullen@uiowa.edu
Garry R Buettner - Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: garry-buettner@uiowa.edu
Resource Type: Journal article
Publication Details: Redox biology, Vol.8(C), pp.298-304
DOI: 10.1016/j.redox.2016.02.004
PMID: 26928133
PMCID: PMC4776628
NLM abbreviation: Redox Biol
ISSN: 2213-2317
eISSN: 2213-2317
Publisher: Netherlands
Grant note: R01 GM073929 / NIGMS NIH HHS P01 HL046925 / NHLBI NIH HHS T32 CA078586 / NCI NIH HHS R01 CA184051 / NCI NIH HHS P30 CA086862 / NCI NIH HHS R01 CA169046 / NCI NIH HHS
Language: English
Date published: 08/2016
Academic Unit: Surgery; Radiation Oncology
Record Identifier: 9984047647002771

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