Journal article
Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D
Cell adhesion & migration, Vol.15(1), pp.224-248
01/01/2021
DOI: 10.1080/19336918.2021.1957527
PMCID: PMC8331046
PMID: 34338608
Abstract
We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.
Details
- Title: Subtitle
- Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D
- Creators
- Deborah J Wessels - Developmental Studies Hybridoma BankClaude Pujol - Developmental Studies Hybridoma BankNikash Pradhan - Developmental Studies Hybridoma BankDaniel F Lusche - Developmental Studies Hybridoma BankLuis Gonzalez - Developmental Studies Hybridoma BankSydney E Kelly - Developmental Studies Hybridoma BankElizabeth M Martin - Developmental Studies Hybridoma BankEdward R Voss - Developmental Studies Hybridoma BankYang-Nim Park - University of IowaMichael Dailey - Developmental Studies Hybridoma BankSonia L Sugg - University of Iowa Hospitals and ClinicsSneha Phadke - University of Iowa Hospitals and ClinicsAmani Bashir - University of Iowa Hospitals and ClinicsDavid R Soll - Developmental Studies Hybridoma Bank
- Resource Type
- Journal article
- Publication Details
- Cell adhesion & migration, Vol.15(1), pp.224-248
- DOI
- 10.1080/19336918.2021.1957527
- PMID
- 34338608
- PMCID
- PMC8331046
- NLM abbreviation
- Cell Adh Migr
- ISSN
- 1933-6918
- eISSN
- 1933-6926
- Publisher
- Taylor & Francis
- Grant note
- DOI: 10.13039/100000054, name: National Cancer Institute, award: P30CA086062; name: Developmental Studies Hybridoma Bank; name: National Cancer Institute, United States, award: P30CA086062; name: National Cancer Institute, United States, award: P30CA086062
- Language
- English
- Date published
- 01/01/2021
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Pathology; Iowa Neuroscience Institute; Surgery; Biology; Internal Medicine
- Record Identifier
- 9984185180602771
Metrics
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