Discordant varicella-zoster virus glycoprotein C expression and localization between cultured cells and human skin vesicles

Johnathan Storlie; John E Carpenter; Wallen Jackson; Charles Grose

doi:10.1016/j.virol.2008.09.031

Back

Discordant varicella-zoster virus glycoprotein C expression and localization between cultured cells and human skin vesicles

Journal article

Open access

Peer reviewed

Discordant varicella-zoster virus glycoprotein C expression and localization between cultured cells and human skin vesicles

Johnathan Storlie, John E Carpenter, Wallen Jackson and Charles Grose

Virology (New York, N.Y.), Vol.382(2), pp.171-181

12/20/2008

DOI: 10.1016/j.virol.2008.09.031

PMCID: PMC2754791

PMID: 18954885

Files and links (1)

url

https://doi.org/10.1016/j.virol.2008.09.031View

Published (Version of record) Open Access

Abstract

Because of its very low titer, varicella-zoster virus (VZV) infectivity is usually transferred by passage of trypsin dispersed infected cells. Previously, we observed that gC biosynthesis was markedly delayed in monolayers inoculated with cell free virus. In this report, we investigated the kinetics of gC expression in more detail and included studies of monolayers inoculated with trypsin dispersed infected cells, the more traditional method of VZV infection. Extensive imaging analyses disclosed that gC was detectable in some inoculum cells, but little gC biosynthesis occurred during the first 48 hpi in the newly infected underlying monolayer. In contrast, during the first 24-48 hpi, expression of VZV gE and gB was easily detectable. Using real-time RT-PCR, we found a delay in accumulation of VZV gC transcripts that paralleled the delay in expression of VZV gC protein. Treatment with hexamethylene bisacetamide (HMBA) increased expression of both gC protein and gC mRNA. HMBA treatment also increased virus titer by 4-fold, but paradoxically reduced plaque size in the titration assay. Finally, we examined skin vesicles from cases of chickenpox and zoster in humans and observed abundant amounts of gC expression. In short, this report documents an unexpected delay in both gC mRNA and protein production under all conditions of VZV infection of cultured cells.

Gene Expression - drug effects

Humans

Acetamides - pharmacology

RNA, Messenger - metabolism

Viral Proteins - metabolism

Giant Cells - virology

Herpesvirus 3, Human - drug effects

RNA, Viral - genetics

Herpesvirus 3, Human - metabolism

Chickenpox - virology

Genes, Viral

Viral Envelope Proteins - metabolism

RNA, Viral - metabolism

Skin - virology

Virus Replication - drug effects

Viral Envelope Proteins - genetics

RNA, Messenger - genetics

Cells, Cultured

Viral Proteins - genetics

Chickenpox - pathology

Herpesvirus 3, Human - genetics

Herpes Zoster - pathology

Herpes Zoster - virology

Herpesvirus 3, Human - pathogenicity

Kinetics

Giant Cells - pathology

Skin - drug effects

Details

Title: Subtitle: Discordant varicella-zoster virus glycoprotein C expression and localization between cultured cells and human skin vesicles
Creators: Johnathan Storlie - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, IA 52242, USA
John E Carpenter
Wallen Jackson
Charles Grose
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.382(2), pp.171-181
DOI: 10.1016/j.virol.2008.09.031
PMID: 18954885
PMCID: PMC2754791
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Grant note: R01 AI089716 / NIAID NIH HHS AI22795 / NIAID NIH HHS R01 AI022795 / NIAID NIH HHS R01 AI022795-20 / NIAID NIH HHS
Language: English
Date published: 12/20/2008
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093225902771

Metrics

14 Record Views

28 Times Cited - Web of Science