Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans

Pawel Bieganowski; Charles Brenner

doi:10.1016/S0092-8674(04)00416-7

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Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans

Journal article

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Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans

Pawel Bieganowski and Charles Brenner

Cell, Vol.117(4), pp.495-502

05/14/2004

DOI: 10.1016/S0092-8674(04)00416-7

PMID: 15137942

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Abstract

NAD+ is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in various reactions, including those that retard aging in experimental systems. Nicotinic acid and nicotinamide were defined as the vitamin precursors of NAD+ in Elvehjem's classic discoveries of the 1930s. The accepted view of eukaryotic NAD+ biosynthesis, that all anabolism flows through nicotinic acid mononucleotide, was challenged experimentally and revealed that nicotinamide riboside is an unanticipated NAD+ precursor in yeast. Nicotinamide riboside kinases from yeast and humans essential for this pathway were identified and found to be highly specific for phosphorylation of nicotinamide riboside and the cancer drug tiazofurin. Nicotinamide riboside was discovered as a nutrient in milk, suggesting that nicotinamide riboside is a useful compound for elevation of NAD+ levels in humans.

Niacinamide - analogs & derivatives

Phosphorylation

Humans

Molecular Sequence Data

Ribavirin - analogs & derivatives

Fungi - genetics

Chromosomes, Human, Pair 9 - genetics

Niacinamide - chemistry

NAD - biosynthesis

Saccharomyces cerevisiae Proteins - isolation & purification

Gene Expression Regulation, Fungal - genetics

Energy Metabolism - genetics

Nucleosides - chemistry

Niacinamide - metabolism

Phosphotransferases (Alcohol Group Acceptor) - isolation & purification

Fungi - enzymology

Intracellular Signaling Peptides and Proteins

Phosphotransferases (Alcohol Group Acceptor) - genetics

Ribavirin - chemistry

Saccharomyces cerevisiae Proteins - genetics

Sequence Homology, Nucleic Acid

Phosphotransferases (Alcohol Group Acceptor) - metabolism

Sequence Homology, Amino Acid

Gene Expression Regulation, Enzymologic - genetics

Saccharomyces cerevisiae Proteins - metabolism

Ribavirin - pharmacology

Evolution, Molecular

Details

Title: Subtitle: Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans
Creators: Pawel Bieganowski - Departments of Genetics and Biochemistry and the Norris Cotton Cancer Center, Dartmouth Medical School, Rubin 733-HB7937, Lebanon, NH 03756, USA
Charles Brenner
Resource Type: Journal article
Publication Details: Cell, Vol.117(4), pp.495-502
DOI: 10.1016/S0092-8674(04)00416-7
PMID: 15137942
NLM abbreviation: Cell
ISSN: 0092-8674
eISSN: 1097-4172
Publisher: United States
Grant note: CA77738 / NCI NIH HHS P01 CA077738-040003 / NCI NIH HHS P01 CA077738 / NCI NIH HHS
Language: English
Date published: 05/14/2004
Academic Unit: Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9983788429102771

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