Journal article
Discovery of new human β-defensins using a genomics-based approach
Gene, Vol.263(1-2), pp.211-218
01/2001
DOI: 10.1016/S0378-1119(00)00569-2
PMID: 11223260
Abstract
Epithelial β-defensins are broad-spectrum cationic antimicrobial peptides that also act as chemokines for adaptive immune cells. In the human genome, all known defensin genes cluster to a <1 Mb region of chromosome 8p22-p23. To identify new defensin genes, the DNA sequence from a contig of large-insert genomic clones from the region containing human β-defensin-2 (HBD-2) was analyzed for the presence of defensin genes. This sequence survey identified a novel β-defensin, termed HBD-3. The HBD-3 gene contains two exons, is located 13 kb upstream from the HBD-2 gene, and it is transcribed in the same direction. A partial HBD-3 cDNA clone was amplified from cDNA derived from IL-1β induced fetal lung tissue. The cDNA sequence encodes for a 67 amino acid peptide that is ∼43% identical to HBD-2 and shares the β-defensin six cysteine motif. By PCR analysis of two commercial cDNA panels, HBD-3 expression was detected in adult heart, skeletal muscle, placenta and in fetal thymus. From RT-PCR experiments, HBD-3 expression was observed in skin, esophagus, gingival keratinocytes, placenta and trachea. Furthermore, in fetal lung explants and gingival keratinocytes, HBD-3 mRNA expression was induced by IL-1β. Additional sequence analysis identified the HE2 (human epididymis secretory protein) gene 17 kb upstream from the HBD-3 gene. One splice variant of this gene (HE2β1) encodes a β-defensin consensus cysteine motif, suggesting it represents a defensin gene product. HE2β1 mRNA expression was detected in gingival keratinocytes and bronchial epithelia using RT-PCR analysis. The discovery of these novel β-defensin genes may allow further understanding of the role of defensins in host immunity at mucosal surfaces.
Details
- Title: Subtitle
- Discovery of new human β-defensins using a genomics-based approach
- Creators
- Hong Peng JiaBrian C SchutteAndreas SchudyRose LinzmeierJanet M GuthmillerGeorgia K JohnsonBrian F TackJoseph P MitrosAndre RosenthalTomas GanzPaul B McCray
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.263(1-2), pp.211-218
- DOI
- 10.1016/S0378-1119(00)00569-2
- PMID
- 11223260
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 01/2001
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Periodontics; Internal Medicine
- Record Identifier
- 9984065976102771
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