Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos

Laird C Sheldahl; Diane C Slusarski; Petra Pandur; Jeffrey R Miller; Michael Kühl; Randall T Moon

doi:10.1083/jcb.200211094

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Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos

Journal article

Open access

Peer reviewed

Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos

Laird C Sheldahl, Diane C Slusarski, Petra Pandur, Jeffrey R Miller, Michael Kühl and Randall T Moon

The Journal of cell biology, Vol.161(4), pp.769-777

05/26/2003

DOI: 10.1083/jcb.200211094

PMCID: PMC2199364

PMID: 12771126

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Abstract

Wnt ligands and Frizzled (Fz) receptors have been shown to activate multiple intracellular signaling pathways. Activation of the Wnt-beta-catenin pathway has been described in greatest detail, but it has been reported that Wnts and Fzs also activate vertebrate planar cell polarity (PCP) and Wnt-Ca2+ pathways. Although the intracellular protein Dishevelled (Dsh) plays a dual role in both the Wnt-beta-catenin and the PCP pathways, its potential involvement in the Wnt-Ca2+ pathway has not been investigated. Here we show that a Dsh deletion construct, XDshDeltaDIX, which is sufficient for activation of the PCP pathway, is also sufficient for activation of three effectors of the Wnt-Ca2+ pathway: Ca2+ flux, PKC, and calcium/calmodulin-dependent protein kinase II (CamKII). Furthermore, we find that interfering with endogenous Dsh function reduces the activation of PKC by Xfz7 and interferes with normal heart development. These data suggest that the Wnt-Ca2+ pathway utilizes Dsh, thereby implicating Dsh as a component of all reported Fz signaling pathways.

Xenopus laevis - embryology

Calcium - metabolism

RNA, Messenger - genetics

Embryo, Nonmammalian - metabolism

Phosphoproteins - metabolism

RNA, Messenger - metabolism

Zebrafish - embryology

Embryo, Nonmammalian - drug effects

Blotting, Western

Adaptor Proteins, Signal Transducing

Animals

Embryo, Nonmammalian - enzymology

In Situ Hybridization

Protein Kinase C - metabolism

Xenopus laevis - metabolism

Zebrafish - metabolism

Xenopus Proteins - metabolism

Dishevelled Proteins

Calcium-Calmodulin-Dependent Protein Kinase Type 2

Calcium-Calmodulin-Dependent Protein Kinases - metabolism

Pertussis Toxin - pharmacology

Details

Title: Subtitle: Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos
Creators: Laird C Sheldahl - Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
Diane C Slusarski
Petra Pandur
Jeffrey R Miller
Michael Kühl
Randall T Moon
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.161(4), pp.769-777
Publisher: United States
DOI: 10.1083/jcb.200211094
PMID: 12771126
PMCID: PMC2199364
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: R01 HD27525 / NICHD NIH HHS R01 HD027525 / NICHD NIH HHS
Language: English
Date published: 05/26/2003
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983991958802771

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