Journal article
Disorganized Innervation and Neuronal Loss in the Inner Ear of Slitrk6-Deficient Mice
PloS one, Vol.4(11), pp.A151-A162
11/11/2009
DOI: 10.1371/journal.pone.0007786
PMCID: PMC2777407
PMID: 19936227
Abstract
Slitrks are type I transmembrane proteins that share conserved leucine-rich repeat domains similar to those in the secreted axonal guidance molecule Slit. They also show similarities to Ntrk neurotrophin receptors in their carboxy-termini, sharing a conserved tyrosine residue. Among 6
Slitrk
family genes in mammals,
Slitrk6
has a unique expression pattern, with strong expression in the sensory epithelia of the inner ear. We generated
Slitrk6
-knockout mice and investigated the development of their auditory and vestibular sensory organs.
Slitrk6
-deficient mice showed pronounced reduction in the cochlear innervation. In the vestibule, the innervation to the posterior crista was often lost, reduced, or sometimes misguided. These defects were accompanied by the loss of neurons in the spiral and vestibular ganglia. Cochlear sensory epithelia from
Slitrk6
-knockout mice have reduced ability in promoting neurite outgrowth of spiral ganglion neurons. Indeed the
Slitrk6
-deficient inner ear showed a mild but significant decrease in the expression of
Bdnf
and
Ntf3
, both of which are essential for the innervation and survival of sensory neurons. In addition, the expression of Ntrk receptors, including their phosphorylated forms was decreased in
Slitrk6
-knockout cochlea. These results suggest that Slitrk6 promotes innervation and survival of inner ear sensory neurons by regulating the expression of trophic and/or tropic factors including neurotrophins from sensory epithelia.
Details
- Title: Subtitle
- Disorganized Innervation and Neuronal Loss in the Inner Ear of Slitrk6-Deficient Mice
- Creators
- Kei-ichi KatayamaAzel ZineMaya OtaYoshifumi MatsumotoTakashi InoueBernd FritzschJun Aruga
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.4(11), pp.A151-A162
- DOI
- 10.1371/journal.pone.0007786
- PMID
- 19936227
- PMCID
- PMC2777407
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; San Francisco, USA
- Alternative title
- Inner Ear Defect in Slitrk6 KO
- Language
- English
- Date published
- 11/11/2009
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070228302771
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