Disposition and Metabolomic Effects of 2,2',5,5'-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure

Amanda Bullert; Xueshu Li; Zhang Chunyun; Kendra Lee; Casey F Pulliam; Brianna S Cagle; Jonathan A Doorn; Aloysius J Klingelhutz; Larry W Robertson; Hans-Joachim Lehmler

doi:10.1016/j.etap.2023.104245

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Disposition and Metabolomic Effects of 2,2',5,5'-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure

Journal article

Peer reviewed

Disposition and Metabolomic Effects of 2,2',5,5'-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure

Amanda Bullert, Xueshu Li, Zhang Chunyun, Kendra Lee, Casey F Pulliam, Brianna S Cagle, Jonathan A Doorn, Aloysius J Klingelhutz, Larry W Robertson and Hans-Joachim Lehmler

Environmental toxicology and pharmacology, Vol.102, 104245

09/2023

DOI: 10.1016/j.etap.2023.104245

PMCID: PMC10562985

PMID: 37572994

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https://pmc.ncbi.nlm.nih.gov/articles/PMC10562985/pdf/nihms-1925652.pdfView

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Abstract

The disposition and toxicity of lower chlorinated PCBs (LC-PCBs) with less than five chlorine substituents have received little attention. This study characterizes the distribution and metabolomic effects of PCB 52, an LC-PCB found in indoor and outdoor air, three weeks after intraperitoneal exposure of female Sprague Dawley rats to 0, 1, 10, or 100mg/kg BW. PCB 52 exposure did not affect overall body weight. Gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis identified PCB 52 in all tissues investigated. Hydroxylated, sulfated, and methylated PCB metabolites, identified using GC-MS/MS and nontarget liquid chromatography-high resolution mass spectrometry (Nt-LCMS), were primarily found in the serum and liver of rats exposed to 100mg/kg BW. Metabolomic analysis revealed minor effects on L-cysteine, glycine, cytosine, sphingosine, thymine, linoleic acid, orotic acid, L-histidine, and erythrose serum levels. Thus, the metabolism of PCB 52 and its effects on the metabolome must be considered in toxicity studies.

dose-response

hydroxylated metabolites, polychlorinated biphenyls, PCB 52

disposition

Details

Title: Subtitle: Disposition and Metabolomic Effects of 2,2',5,5'-Tetrachlorobiphenyl in Female Rats Following Intraperitoneal Exposure
Creators: Amanda Bullert - University of Iowa
Xueshu Li - University of Iowa
Zhang Chunyun - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA 52242, USA
Kendra Lee - University of Iowa
Casey F Pulliam - University of Iowa
Brianna S Cagle - University of Iowa
Jonathan A Doorn - University of Iowa
Aloysius J Klingelhutz - University of Iowa
Larry W Robertson - University of Iowa
Hans-Joachim Lehmler - University of Iowa
Resource Type: Journal article
Publication Details: Environmental toxicology and pharmacology, Vol.102, 104245
DOI: 10.1016/j.etap.2023.104245
PMID: 37572994
PMCID: PMC10562985
NLM abbreviation: Environ Toxicol Pharmacol
ISSN: 1382-6689
eISSN: 1872-7077
Grant note: DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P30ES005605, P42ES013661, R01ES014901, R01ES031098; DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Electronic publication date: 08/10/2023
Date published: 09/2023
Academic Unit: Microbiology and Immunology; Occupational and Environmental Health; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Radiation Oncology; Iowa Superfund Research Program; Medicinal and Natural Products Chemistry
Record Identifier: 9984455299502771

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