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Disruption of Phosphatidylcholine Monolayers and Bilayers by Perfluorobutane Sulfonate
Journal article   Open access   Peer reviewed

Disruption of Phosphatidylcholine Monolayers and Bilayers by Perfluorobutane Sulfonate

E. Davis Oldham, Wei Xie, Amir M Farnoud, Jennifer Fiegel and Hans-Joachim Lehmler
The journal of physical chemistry. B, Vol.116(33), pp.9999-10007
08/23/2012
DOI: 10.1021/jp304412p
PMCID: PMC3464004
PMID: 22834732
url
https://www.ncbi.nlm.nih.gov/pmc/articles/3464004View
Open Access

Abstract

Perfluoroalkyl acids (PFAAs) are persistent environmental contaminants resistant to biological and chemical degradation due to the presence of carbon-fluorine bonds. These compounds exhibit developmental toxicity in vitro and in vivo. The mechanisms of toxicity may involve partitioning into lipid bilayers. We investigated the interaction between perfluorobutane sulfonate (PFBS), an emerging PFAA, and model phosphatidylcholine (PC) lipid assemblies (i.e., dimyristoyl-, dipalmitoyl- and distearylphosphatidylcholine) using fluorescence anisotropy and Langmuir monolayer techniques. PFBS decreased the transition temperature and transition width of PC bilayers. The apparent membrane partition coefficients ranged from 4.9 × 10 2 to 8.2 × 10 2 . The effects on each PC were comparable. The limiting molecular area of PC monolayers increased and the surface pressure at collapse decreased in a concentration-dependent manner. The compressibility of all three PCs was decreased by PFBS. In summary, PFBS disrupted different model lipid assemblies indicating potential for PFBS to be a human toxicant. However the effects of PFBS are not as pronounced as those seen with longer chain PFAAs.
model lipid assemblies cooperativity Perfluoroalkyl acids membrane fluidity

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