Journal article
Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs
Nature communications, Vol.11(1), pp.462-462
01/23/2020
DOI: 10.1038/s41467-019-14266-z
PMCID: PMC6978526
PMID: 31974374
Abstract
Human induced pluripotent stem cells (hiPSCs) are a powerful model of neural differentiation and maturation. We present a hiPSC transcriptomics resource on corticogenesis from 5 iPSC donor and 13 subclonal lines across 9 time points over 5 broad conditions: self-renewal, early neuronal differentiation, neural precursor cells (NPCs), assembled rosettes, and differentiated neuronal cells. We identify widespread changes in the expression of both individual features and global patterns of transcription. We next demonstrate that co-culturing human NPCs with rodent astrocytes results in mutually synergistic maturation, and that cell type-specific expression data can be extracted using only sequencing read alignments without cell sorting. We lastly adapt a previously generated RNA deconvolution approach to single-cell expression data to estimate the relative neuronal maturity of iPSC-derived neuronal cultures and human brain tissue. Using many public datasets, we demonstrate neuronal cultures are maturationally heterogeneous but contain subsets of neurons more mature than previously observed.
Details
- Title: Subtitle
- Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs
- Creators
- Emily E Burke - Lieber Institute for Brain DevelopmentJoshua G Chenoweth - Lieber Institute for Brain DevelopmentJoo Heon Shin - Lieber Institute for Brain DevelopmentLeonardo Collado-Torres - Lieber Institute for Brain DevelopmentSuel-Kee Kim - Lieber Institute for Brain DevelopmentNicola Micali - Lieber Institute for Brain DevelopmentYanhong Wang - Lieber Institute for Brain DevelopmentCarlo Colantuoni - Lieber Institute for Brain DevelopmentRichard E Straub - Lieber Institute for Brain DevelopmentDaniel J Hoeppner - Lieber Institute for Brain DevelopmentHuei-Ying Chen - Lieber Institute for Brain DevelopmentAlana Sellers - Lieber Institute for Brain DevelopmentKamel Shibbani - Lieber Institute for Brain DevelopmentGregory R Hamersky - Lieber Institute for Brain DevelopmentMarcelo Diaz Bustamante - Lieber Institute for Brain DevelopmentBaDoi N Phan - Lieber Institute for Brain DevelopmentWilliam S Ulrich - Lieber Institute for Brain DevelopmentCristian Valencia - Lieber Institute for Brain DevelopmentAmritha Jaishankar - Lieber Institute for Brain DevelopmentAmanda J Price - Lieber Institute for Brain DevelopmentAnandita Rajpurohit - Lieber Institute for Brain DevelopmentStephen A Semick - Lieber Institute for Brain DevelopmentRoland W Bürli - AstraZenecaJames C Barrow - Lieber Institute for Brain DevelopmentDaniel J Hiler - Lieber Institute for Brain DevelopmentStephanie C Page - Lieber Institute for Brain DevelopmentKeri Martinowich - Johns Hopkins MedicineThomas M Hyde - Lieber Institute for Brain DevelopmentJoel E Kleinman - Lieber Institute for Brain DevelopmentKaren F Berman - Office of Extramural ResearchJose A Apud - Office of Extramural ResearchAlan J Cross - AstraZenecaNicholas J Brandon - AstraZenecaDaniel R Weinberger - Johns Hopkins UniversityBrady J Maher - Johns Hopkins MedicineRonald D G McKay - Lieber Institute for Brain DevelopmentAndrew E Jaffe - Johns Hopkins Medicine
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.11(1), pp.462-462
- DOI
- 10.1038/s41467-019-14266-z
- PMID
- 31974374
- PMCID
- PMC6978526
- ISSN
- 2041-1723
- eISSN
- 2041-1723
- Language
- English
- Date published
- 01/23/2020
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984702945702771
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