Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors

T M Gallagher; M J Buchmeier; S Perlman

doi:10.1007/978-1-4615-2996-5_43

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Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors

Journal article

Open access

Peer reviewed

Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors

T M Gallagher, M J Buchmeier and S Perlman

Advances in experimental medicine and biology, Vol.342, pp.279-284

1993

DOI: 10.1007/978-1-4615-2996-5_43

PMID: 8209743

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Abstract

In this report, we demonstrate the syncytial spread of MHV4 (strain JHM) infection through non-murine cell cultures which lack a specific MHV4 receptor and are therefore resistant to infection by free virions. This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay. Receptor-independent syncytium formation induced by cells expressing the MHV4 spike (S) from recombinant vaccinia viruses (VV) indicated that spread was mediated by this coronavirus glycoprotein. We conclude that the S protein of MHV4 is so potently fusogenic that it does not require prior binding to a virus-specific surface receptor to induce fusion of closely-opposed plasma membranes.

Viral Envelope Proteins - physiology

Cell Line - microbiology

Cricetinae

Species Specificity

Membrane Glycoproteins - chemistry

Cytopathogenic Effect, Viral

Rats

Recombinant Fusion Proteins - metabolism

Kidney

Murine hepatitis virus - physiology

Receptors, Virus - physiology

Spike Glycoprotein, Coronavirus

Cell Fusion

Tumor Cells, Cultured - microbiology

Membrane Glycoproteins - physiology

Animals

Receptors, Virus - deficiency

Virus Replication

Viral Envelope Proteins - chemistry

Mesocricetus

Genetic Vectors

Astrocytoma

Details

Title: Subtitle: Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors
Creators: T M Gallagher - Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037
M J Buchmeier
S Perlman
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.342, pp.279-284
DOI: 10.1007/978-1-4615-2996-5_43
PMID: 8209743
NLM abbreviation: Adv Exp Med Biol
ISSN: 0065-2598
Publisher: United States
Grant note: NS 22347 / NINDS NIH HHS NS 12428 / NINDS NIH HHS NS 24401 / NINDS NIH HHS
Language: English
Date published: 1993
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777352402771

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