Dissociation between vasopressin and oxytocin mRNA and peptide secretion after AV3V lesions

Mariana Morris; Maria Jose Alves Rocha; Laura J Sim; Alan Kim Johnson; Michael F Callahan

doi:10.1152/ajpregu.1994.267.6.r1640

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Dissociation between vasopressin and oxytocin mRNA and peptide secretion after AV3V lesions

Journal article

Peer reviewed

Dissociation between vasopressin and oxytocin mRNA and peptide secretion after AV3V lesions

Mariana Morris, Maria Jose Alves Rocha, Laura J Sim, Alan Kim Johnson and Michael F Callahan

The American journal of physiology, Vol.267(6 Pt 2), pp.R1640-1645

12/1994

DOI: 10.1152/ajpregu.1994.267.6.r1640

PMID: 7810775

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Abstract

The effect of hypertonic NaCl consumption on vasopressin (VP) and oxytocin (OT) mRNA levels and plasma and pituitary peptides was evaluated in rats with sham or anterior ventral third ventricular (AV3V) lesions. Rats were given tap water or 2% NaCl for 4 days. Because the rats with lesions drank significantly less salt solution than the controls (78.8 +/- 17.4 vs. 205.5 +/- 37.8 ml/4 days), a second control group was included in which saline intake was matched to the lesioned group. AV3V rats showed a deficit in the peptide response to the osmotic stimulus. There was no increase in plasma VP or OT or decrease in posterior pituitary peptide content in the face of an extreme hypernatremia: plasma sodium of 180.1 +/- 4.2 meq/l. Evaluation of mRNA changes by means of in situ hybridization showed that animals with lesions responded to the salt challenge with increases in hypothalamic VP and OT mRNA levels. There were significant increases in paraventricular and supraoptic OT mRNA and paraventricular VP mRNA in the lesioned group. The salt-matched control group showed no changes in peptide mRNA levels. These results demonstrate that AV3V lesions produce an impairment of the salt-neuroendocrine reflex but a persistence of the peptide mRNA response. Differences in control mechanisms must account for this dissociation between peptide mRNA expression and peptide secretion.

Animals

Base Sequence

Cerebral Ventricles - physiology

Cerebral Ventricles - surgery

In Situ Hybridization

Male

Molecular Sequence Data

Neuropeptides - secretion

Oxytocin - genetics

Paraventricular Hypothalamic Nucleus - metabolism

Pituitary Gland, Posterior - metabolism

Rats

Rats, Sprague-Dawley

RNA, Messenger - metabolism

Sodium - blood

Sodium Chloride - administration & dosage

Supraoptic Nucleus - metabolism

Vasopressins - genetics

Details

Title: Subtitle: Dissociation between vasopressin and oxytocin mRNA and peptide secretion after AV3V lesions
Creators: Mariana Morris - Department of Physiology and Pharmacology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27157
Maria Jose Alves Rocha
Laura J Sim
Alan Kim Johnson
Michael F Callahan
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.267(6 Pt 2), pp.R1640-1645
DOI: 10.1152/ajpregu.1994.267.6.r1640
PMID: 7810775
ISSN: 0002-9513
eISSN: 2163-5773
Grant note: HL-14338 / NHLBI NIH HHS HL-43178 / NHLBI NIH HHS HL-44546 / NHLBI NIH HHS
Language: English
Date published: 12/1994
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
Record Identifier: 9984213406602771

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