Dissociation of experimentally induced drinking behavior by ibotenate injection into the median preoptic nucleus

J. Thomas Cunningham; Margaret J Sullivan; Gaylen L Edwards; Roxanna Farinpour; T G Beltz; Alan Kim Johnson

doi:10.1016/0006-8993(91)90183-V

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Dissociation of experimentally induced drinking behavior by ibotenate injection into the median preoptic nucleus

Journal article

Peer reviewed

Dissociation of experimentally induced drinking behavior by ibotenate injection into the median preoptic nucleus

J. Thomas Cunningham, Margaret J Sullivan, Gaylen L Edwards, Roxanna Farinpour, T G Beltz and Alan Kim Johnson

Brain research, Vol.554(1-2), pp.153-158

07/19/1991

DOI: 10.1016/0006-8993(91)90183-V

PMID: 1933297

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Abstract

Adult male Sprague-Dawley rats were injected with either ibotenic acid (5 micrograms/1 microliter) or vehicle in the ventral median preoptic nucleus. The injections were given during either methoxyflurane or ketamine anesthesia. None of the rats injected with ibotenic acid were adipsic after surgery. After at least one week of recovery, all rats were tested for drinking responses to angiotensin II (1.5 and 3 mg/kg s.c.) and hypertonic saline (3% and 12% 1 ml/100 g b.wt.). Rats injected with ibotenic acid during methoxyflurane anesthesia drank significantly less than the vehicle injected control group in tests with both doses of angiotensin II and both concentrations of hypertonic saline. The rats that were injected with ibotenic acid during ketamine anesthesia drank significantly less than the control group when tested with 3% hypertonic saline but not when tested with angiotensin II. Histological examination of the injection sites indicated significant reductions in the volume of the median preoptic nucleus only in rats that had been injected with ibotenic acid during methoxyflurane anesthesia. The results are consistent with the interpretation that the drinking response to angiotensin II is dependent upon the integrity of neurons with postsynaptic N-methyl-D-aspartate receptors within the median preoptic nucleus.

Analysis of Variance

Angiotensin II - pharmacology

Animals

Dose-Response Relationship, Drug

Drinking Behavior - drug effects

Ibotenic Acid - administration & dosage

Ibotenic Acid - pharmacology

Male

Microinjections

Preoptic Area - drug effects

Preoptic Area - physiology

Rats

Rats, Inbred Strains

Reference Values

Saline Solution, Hypertonic

Details

Title: Subtitle: Dissociation of experimentally induced drinking behavior by ibotenate injection into the median preoptic nucleus
Creators: J. Thomas Cunningham - Department of Psychology, University of Iowa, Iowa City 52242
Margaret J Sullivan
Gaylen L Edwards
Roxanna Farinpour
T G Beltz
Alan Kim Johnson
Resource Type: Journal article
Publication Details: Brain research, Vol.554(1-2), pp.153-158
DOI: 10.1016/0006-8993(91)90183-V
PMID: 1933297
ISSN: 0006-8993
eISSN: 1872-6240
Grant note: HL33447 / NHLBI NIH HHS HL14558 / NHLBI NIH HHS HL33796 / NHLBI NIH HHS
Language: English
Date published: 07/19/1991
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
Record Identifier: 9984213275202771

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