Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor

Christoph Straub; Yoav Noam; Toshihiro Nomura; Miwako Yamasaki; Dan Yan; Herman B Fernandes; Ping Zhang; James R Howe; Masahiko Watanabe; Anis Contractor; Susumu Tomita

doi:10.1016/j.celrep.2016.05.093

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Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor

Journal article

Open access

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Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor

Christoph Straub, Yoav Noam, Toshihiro Nomura, Miwako Yamasaki, Dan Yan, Herman B Fernandes, Ping Zhang, James R Howe, Masahiko Watanabe, Anis Contractor, …

Cell reports (Cambridge), Vol.16(2), pp.531-544

07/12/2016

DOI: 10.1016/j.celrep.2016.05.093

PMCID: PMC4963241

PMID: 27346345

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https://doi.org/10.1016/j.celrep.2016.05.093View

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Abstract

Synaptic communication between neurons requires the precise localization of neurotransmitter receptors to the correct synapse type. Kainate-type glutamate receptors restrict synaptic localization that is determined by the afferent presynaptic connection. The mechanisms that govern this input-specific synaptic localization remain unclear. Here, we examine how subunit composition and specific subunit domains contribute to synaptic localization of kainate receptors. The cytoplasmic domain of the GluK2 low-affinity subunit stabilizes kainate receptors at synapses. In contrast, the extracellular domain of the GluK4/5 high-affinity subunit synergistically controls the synaptic specificity of kainate receptors through interaction with C1q-like proteins. Thus, the input-specific synaptic localization of the native kainate receptor complex involves two mechanisms that underlie specificity and stabilization of the receptor at synapses.

Animals

Cerebellum - cytology

Cerebellum - metabolism

Hippocampus - cytology

Hippocampus - metabolism

Mice, Transgenic

Protein Domains

Protein Stability

Protein Subunits - chemistry

Protein Subunits - physiology

Protein Transport

Receptors, Kainic Acid - chemistry

Receptors, Kainic Acid - physiology

Synapses - metabolism

Details

Title: Subtitle: Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor
Creators: Christoph Straub - Yale University
Yoav Noam - Yale University
Toshihiro Nomura - Northwestern University
Miwako Yamasaki - Hokkaido University
Dan Yan - Yale University
Herman B Fernandes - Northwestern University
Ping Zhang - Yale University
James R Howe - Yale University
Masahiko Watanabe - Hokkaido University
Anis Contractor - Northwestern University
Susumu Tomita - Yale University
Resource Type: Journal article
Publication Details: Cell reports (Cambridge), Vol.16(2), pp.531-544
DOI: 10.1016/j.celrep.2016.05.093
PMID: 27346345
PMCID: PMC4963241
ISSN: 2211-1247
eISSN: 2211-1247
Grant note: R01 MH099114 / NIMH NIH HHS UL1 TR001863 / NCATS NIH HHS F32 NS093952 / NINDS NIH HHS R01 MH085080 / NIMH NIH HHS
Language: English
Date published: 07/12/2016
Academic Unit: Surgery
Record Identifier: 9984322764802771

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